cDNA Clone PIK4CA1

• Plasmid: pGEX6P-1-PIK4CA1
• Parent Plasmid: pGEX6P1
• Expressed: GST PIK4CA1
• Genbank: NP_002641
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGCAGAA
ATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCT
CGGAAGCAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTAC
ATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAG
CTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCC
TGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATC
GGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATGCCGA
TGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACT
CCACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGA
GAGAAGCGGCTGCGTGAAGACATAAGCATCATGATTAAATTTTGGACCGC
CATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCCCAG
ATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAA
CAAGCCACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTC
CACCATCTCCAAGAAATCAGGCATGTCTAAGAAAACCAACCGGGGCTCCC
AGCTGCACAAATACTACATGAAGCGCAGGACGCTGCTGCTGTCCCTGCTG
GCCACTGAGATCGAGCGTCTCATCACATGGTACAACCCGCTGTCAGCCCC
GGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGGAGAT
CTAAGTACATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTC
GCCTGGAGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAA
GAACACAGAAGCCATTGGGAACGAAGTGACCCGTCTCGTTCGGTTGGACC
CGGGAGCCGTTAGTGATGTGCCTGAAGCAATCAAGTTCCTGGTCACCTGG
CACACCATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTGTGCTGGGC
GCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTACCCGC
CGCACCCTCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCT
CCGGACGCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCCCTCAGGTA
CGACAAGATGGGCTATGTGCGGGAGTATATTCTGTGGGCAGCGTCTAAAT
CCCAGCTTCTGGCACACCAGTTCATCTGGAACATGAAGACTAACATTTAT
CTAGATGAAGAGGGCCACCAGAAAGACCCTGACATCGGCGACCTCCTGGA
TCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAGGACT
TTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCT
ATCATCAAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTC
GGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACC
CTGAGGCCATTGTGCTGGACATCGACTACAAGTCTGGGACCCCGATGCAG
AGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATGTGG
AGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCCGAGG
ATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCA
GCCATCTTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCA
GATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTCGGCCTGGACCTCT
TTGTTTTTCCCTACCGCGTGGTGGCCACTGCCCCTGGGTGCGGGGTGATC
GAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTGGGCCGCCAGACAGA
CTTCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCCACTC
TGGCCTTCCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTAC
AGCCTCCTGCTGTTCCTGCTGCAGATCAAGGACAGACACAACGGCAACAT
TATGCTGGACAAGAAGGGTCATATCATCCACATCGACTTTGGCTTCATGT
TTGAAAGCTCGCCGGGCGGCAATCTCGGCTGGGAACCCGACATCAAGCTG
ACGGATGAGATGGTGATGATCATGGGGGGCAAGATGGAGGCCACACCCTT
CAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCT
ACATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTG
CCCTGTTTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGGTTTAGCCC
CAACATGACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGA
GCTGCTTCCTCAGCAACAGGAGCCGGACCTACGACATGATCCAGTACTAT
CAGAATGACATCCCCTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMREMAGAWHMTVEQKFGLF
SAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQV
EIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN
ATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSD
KKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISK
KSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELEL
DQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEA
IGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDP
PTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMG
YVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVE
EITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSE
VKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSEL
EKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDL
FKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMY
DYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDK
KGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFM
EMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTE
REAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32666
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 123803 Daltons