cDNA Clone ETNK2

• Plasmid: pMalP ETNK2
• Parent Plasmid: pMAL6P
• Expressed: MBP ETNK2
• Genbank: NP_060678
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCTGTGCCCCCTTCGGCCCCTCAGCCGCGCGCGTCCTTTCA
CCTGAGGAGGCACACGCCTTGCCCGCAGTGCTCATGGGGCATGGAGGAGA
AGGCGGCGGCCAGCGCCAGCTGCCGGGAGCCGCCGGGCCCCCCGAGGGCC
GCCGCCGTCGCGTACTTCGGCATTTCCGTGGACCCGGACGACATCCTTCC
CGGGGCCCTGCGCCTCATCCAGGAGCTGCGGCCGCATTGGAAACCCGAGC
AAGTTCGGACCAAGCGCTTCACGGATGGCATCACCAACAAGCTGGTGGCC
TGCTATGTGGAGGAGGACATGCAGGACTGCGTGCTGGTCCGGGTGTATGG
GGAGCGGACGGAGCTGCTGGTGGACCGGGAGAATGAGGTCAGAAACTTCC
AGCTGCTGCGAGCACACAGCTGTGCCCCCAAACTCTACTGCACCTTCCAG
AATGGGCTGTGCTATGAGTACATGCAGGGTGTGGCCCTGGAGCCTGAGCA
CATCCGTGAGCCCCGGCTTTTCAGGTTAATCGCCTTAGAAATGGCAAAGA
TTCATACTATCCACGCCAACGGCAGCCTGCCCAAGCCCATCCTCTGGCAC
AAGATGCACAATTATTTCACGCTTGTGAAGAACGAGATCAACCCCAGCCT
TTCTGCAGATGTCCCTAAGGTAGAGGTGTTGGAACGGGAGCTGGCCTGGC
TGAAGGAGCATCTGTCCCAGCTGGAGTCCCCTGTGGTGTTTTGTCACAAT
GACCTGCTCTGCAAGAATATCATCTATGACAGCATCAAAGGTCACGTGCG
GTTCATTGACTATGAATATGCTGGCTACAACTACCAAGCTTTTGACATTG
GCAACCATTTCAATGAGTTTGCAGGCGTGAATGAGGTGGATTACTGCCTG
TACCCGGCGCGGGAGACCCAGCTGCAGTGGCTGCACTACTACCTGCAGGC
ACAAAAGGGGATGGCCGTGACCCCCAGGGAGGTGCAAAGGCTCTACGTGC
AAGTCAACAAGTTTGCCCTGGCGTCTCACTTCTTCTGGGCTCTCTGGGCC
CTCATCCAGAACCAGTACTCCACCATCGACTTTGATTTCCTCAGGTACGC
AGTGATCCGATTCAACCAGTACTTCAAGGTGAAGCCTCAAGCGTCAGCCT
TGGAGATGCCAAAGTGAGGATCC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMAVPPSAPQPRASFHLRRHTPC
PQCSWGMEEKAAASASCREPPGPPRAAAVAYFGISVDPDDILPGALRLIQ
ELRPHWKPEQVRTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTELLV
DRENEVRNFQLLRAHSCAPKLYCTFQNGLCYEYMQGVALEPEHIREPRLF
RLIALEMAKIHTIHANGSLPKPILWHKMHNYFTLVKNEINPSLSADVPKV
EVLERELAWLKEHLSQLESPVVFCHNDLLCKNIIYDSIKGHVRFIDYEYA
GYNYQAFDIGNHFNEFAGVNEVDYCLYPARETQLQWLHYYLQAQKGMAVT
PREVQRLYVQVNKFALASHFFWALWALIQNQYSTIDFDFLRYAVIRFNQY
FKVKPQASALEMPK

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32628
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 91517 Daltons