cDNA Clone Acylglycerol Kinase

• Plasmid: pMalP Acylglycerol kinase
• Parent Plasmid: pMAL6P
• Expressed: MBP AGK
• Genbank: AAH22777.1
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGACGGTGTTCTTTAAAACGCTTCGAAATCACTGGAAGAAAAC
TACAGCTGGGCTCTGCCTGCTGACCTGGGGAGGCCATTGGCTCTATGGAA
AACACTGTGATAACCTCCTAAGGAGAGCAGCCTGTCAAGAAGCTCAGGTG
TTTGGCAATCAACTCATTCCTCCCAATGCACAAGTGAAGAAGGCCACTGT
TTTTCTCAATCCTGCAGCTTGCAAAGGAAAAGCTAGGACTCTATTTGAAA
AAAATGCTGCCCCGATTTTACATTTATCTGGCATGGATGTGACTATTGTT
AAGACAGATTATGAGGGACAAGCCAAGAAACTCCTGGAACTGATGGAAAA
CACGGATGTGATCATTGTTGCAGGAGGAGATGGGACACTGCAGGAGGTTG
TTACTGGTGTTCTTCGACGAACAGATGAGGCTACCTTCAGTAAGATTCCC
ATTGGATTTATCCCACTGGGAGAGACCAGTAGTTTGAGTCATACCCTCTT
TGCCGAAAGTGGAAACAAAGTCCAACATATTACTGATGCCACACTTGCCA
TTGTGAAAGGAGAGACAGTTCCACTTGATGTCTTGCAGATCAAGGGTGAA
AAGGAACAGCCTGTATTTGCAATGACCGGCCTTCGATGGGGATCTTTCAG
AGATGCTGGCGTCAAAGTTAGCAAGTACTGGTATCTTGGGCCTCTAAAAA
TCAAAGCAGCCCACTTTTTCAGCACTCTTAAGGAGTGGCCTCAGACTCAT
CAAGCCTCTATCTCATACACGGGACCTACAGAGAGACCTCCCAATGAACC
AGAGGAGACCCCTGTACAAAGGCCTTCTTTGTACAGGAGAATATTACGAA
GGCTTGCGTCCTACTGGGCACAACCACAGGATGCCCTTTCCCAAGAGGTG
AGCCCGGAGGTCTGGAAAGATGTGCAGCTGTCCACCATTGAACTGTCCAT
CACAACACGGAATAATCAGCTTGACCCGACAAGCAAAGAAGATTTTCTGA
ATATCTGCATTGAACCTGACACCATCAGCAAAGGAGACTTTATAACTATA
GGAAGTCGAAAGGTGAGAAACCCCAAGCTGCACGTGGAGGGCACGGAGTG
TCTCCAAGCCAGCCAGTGCACTTTGCTTATCCCGGAGGGAGCAGGGGGCT
CTTTTAGCATTGACAGTGAGGAGTATGAAGCGATGCCTGTGGAGGTGAAA
CTGCTCCCCAGGAAGCTGCAGTTCTTCTGTGATCCTAGGAAGAGAGAACA
GATGCTCACAAGCCCCACCCAGTGAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMTVFFKTLRNHWKKTTAGLCLL
TWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPAAC
KGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVA
GGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGETSSLSHTLFAESGNKV
QHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVS
KYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQR
PSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQL
DPTSKEDFLNICIEPDTISKGDFITIGSRKVRNPKLHVEGTECLQASQCT
LLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSPTQ

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32601
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 93873 Daltons