cDNA Clone CK1 epsilon

• Synonyms: CSNK1E
• Plasmid: pET15 6His SUMO CK1 epsilon
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His SUMO CK1 epsilon
• Genbank: NP_003343
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

gcggccgcgatggagctacgtgtggggaacaagtaccgcctgggacggaagatcgggagcgggtccttcggagatatctacctgggtgccaacatcgcctctggtgaggaagtcgccatcaagctggagtgtgtgaagacaaagcacccccagctgcacatcgagagcaagttctacaagatgatgcagggtggcgtggggatcccgtccatcaagtggtgcggagctgagggcgactacaacgtgatggtcatggagctgctggggcctagcctcgaggacctgttcaacttctgttcccgcaaattcagcctcaagacggtgctgctcttggccgaccagatgatcagccgcatcgagtatatccactccaagaacttcatccaccgggacgtcaagcccgacaacttcctcatggggctggggaagaagggcaacctggtctacatcatcgacttc
ggcctggccaagaagtaccgggacgcccgcacccaccagcacattcccta
ccgggaaaacaagaacctgaccggcacggcccgctacgcttccatcaaca
cgcacctgggcattgagcaaagccgtcgagatgacctggagagcctgggc
tacgtgctcatgtacttcaacctgggctccctgccctggcaggggctcaa
agcagccaccaagcgccagaagtatgaacggatcagcgagaagaagatgt
caacgcccatcgaggtcctctgcaaaggctatccctccgaattctcaaca
tacctcaacttctgccgctccctgcggtttgacgacaagcccgactactc
ttacctacgtcagctcttccgcaacctcttccaccggcagggcttctcct
atgactacgtctttgactggaacatgctgaaattcggtgcagcccggaat
cccgaggatgtggaccgggagcggcgagaacacgaacgcgaggagaggat
ggggcagctacgggggtccgcgacccgagccctgccccctggcccaccca
cgggggccactgccaaccggctccgcagtgccgccgagcccgtggcttcc
acgccagcctcccgcatccagccggctggcaatacttctcccagagcgat
ctcgcgggtcgaccgggagaggaaggtgagtatgaggctgcacaggggtg
cgcccgccaacgtctcctcctcagacctcactgggcggcaagaggtctcc
cggatcccagcctcacagacaagtgtgccatttgaccatctcgggaagtg
agcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVDGAAAMELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEV
AIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELL
GPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFL
MGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTH
LGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMST
PIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYD
YVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTG
ATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAP
ANVSSSDLTGRQEVSRIPASQTSVPFDHLGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32599
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS + Sumo1 tag for bacterial expression; ; Estimated MW of expressed protein : 60269 Daltons; Parental Plasmid DU32587