cDNA Clone DDB2

• Plasmid: pGEX6P-1-DDB2
• Parent Plasmid: pGEX6P1
• Expressed: GST DDB2
• Genbank: NM_000107.1 GI:4557514
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggctcccaagaaacgcccagaaacccagaagacctccgagat
tgtattacgccccaggaacaagaggagcaggagtcccctggagctggagc
ccgaggccaagaagctctgtgcgaagggctccggtcctagcagaagatgt
gactcagactgcctctgggtggggctggctggcccacagatcctgccacc
atgccgcagcatcgtcaggaccctccaccagcataagctgggcagagctt
cctggccatctgtccagcaggggctccagcagtcctttttgcacactctg
gattcttaccggatattacaaaaggctgccccctttgacaggagggctac
atccttggcgtggcacccaactcaccccagcaccgtggctgtgggttcca
aagggggagatatcatgctctggaattttggcatcaaggacaaacccacc
ttcatcaaagggattggagctggagggagcatcactgggctgaagtttaa
ccctctcaataccaaccagttttacgcctcctcaatggagggaacaacta
ggctgcaagactttaaaggcaacattctacgagtttttgccagctcagac
accatcaacatctggttttgtagcctggatgtgtctgctagtagccgaat
ggtggtcacaggagacaacgtggggaacgtgatcctgctgaacatggacg
gcaaagagctttggaatctcagaatgcacaaaaagaaagtgacgcatgtg
gccctgaacccatgctgtgattggttcctggccacagcctccgtagatca
aacagtgaaaatttgggacctgcgccaggttagagggaaagccagcttcc
tctactcgctgccgcacaggcatcctgtcaacgcagcttgtttcagtccc
gatggagcccggctcctgaccacggaccagaagagcgagatccgagttta
ctctgcttcccagtgggactgccccctgggcctgatcccgcaccctcacc
gtcacttccagcacctcacacctatcaaggcagcctggcatcctcgctac
aacctcattgttgtgggccgatacccagatcctaatttcaaaagttgtac
cccttatgaattgaggacgatcgacgtgttcgatggaaactcagggaaga
tgatgtgtcagctctatgacccagaatcttctggcatcagttcgcttaat
gaattcaatcccatgggggacacgctggcctctgcaatgggttaccacat
tctcatctggagccaggaggaagccaggacacggaagtgaggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAPKKRPETQKTSEIVLRP
RNKRSRSPLELEPEAKKLCAKGSGPSRRCDSDCLWVGLAGPQILPPCRSI
VRTLHQHKLGRASWPSVQQGLQQSFLHTLDSYRILQKAAPFDRRATSLAW
HPTHPSTVAVGSKGGDIMLWNFGIKDKPTFIKGIGAGGSITGLKFNPLNT
NQFYASSMEGTTRLQDFKGNILRVFASSDTINIWFCSLDVSASSRMVVTG
DNVGNVILLNMDGKELWNLRMHKKKVTHVALNPCCDWFLATASVDQTVKI
WDLRQVRGKASFLYSLPHRHPVNAACFSPDGARLLTTDQKSEIRVYSASQ
WDCPLGLIPHPHRHFQHLTPIKAAWHPRYNLIVVGRYPDPNFKSCTPYEL
RTIDVFDGNSGKMMCQLYDPESSGISSLNEFNPMGDTLASAMGYHILIWS
QEEARTRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_000107.1 CDS: ; c1017t (silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 74686 Daltons