cDNA Clone USP7

• Plasmid: pEBG6P-USP7 150-550
• Parent Plasmid: pEBG6P
• Expressed: GST USP7 150-550
• Genbank: AK302912.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGA
TTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGA
AAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCG
GATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGT
CGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGA
CGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACC
GAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTT
CTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAA
AGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAG
GAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCAC
CTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCT
ATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTAT
TATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATT
TGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACG
CTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACA
TTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCA
GACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGT
TACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAAT
TATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACA
TTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTG
ATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAAT
TATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTA
CATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGG
TCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAG
AAAAGGATCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSFSRRISHLFFHKE
NDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTG
YVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQR
VFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMK
GTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFE
SFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYD
PQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHG
GHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTN
AYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32584
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 73807 Daltons