cDNA Clone PIP5K1B

• Plasmid: pGEX6P-2-PIP5K1B
• Parent Plasmid: pGEX6P2
• Expressed: GST PIP5K1B
• Genbank: AAH30587.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAA
ACAAAATGAAGAAAAAACCTATAAAAAGACACAAGTGAAAAACCAAATCC
TGTCTCGTTTACCCAAGATTCCGTGTACATTCATTCAAGCATGGACCAAC
ACAGAGATGATTACGTTGCTAGCCTGTTATTACTTCAGATCAGTTTCAAC
TGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAG
TGGGTAATCTCACTTCCAAGCCAGAACGAGATGTTCTTATGCAAGACTTT
TATGTGGTGGAAAGTGTGTTCCTACCCAGCGAAGGGAGCAATCTGACCCC
AGCACATCACTACCCAGACTTTAGATTTAAGACATACGCTCCATTAGCAT
TCCGATATTTCAGAGAACTTTTTGGTATCAAGCCTGATGATTACTTGTAT
TCCATCTGCAGTGAACCTCTAATAGAACTGTCTAACCCTGGAGCCAGTGG
ATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAAACAGTTC
AGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATG
AATTTAAACCAGAATCCAAGGACTCTTTTGCCAAAATTTTACGGACTGTA
TTGTATGCAATCAGGAGGCATTAATATCAGGATTGTGGTGATGAACAACG
TTTTGCCACGCTCCATGAGAATGCACTTTACATATGACTTGAAAGGCTCA
ACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAATCCAACCCCAC
ATTTAAGGACTTAGATTTCCTGCAAGACATGCACGAAGGGTTGTATTTTG
ATACGGAAACATACAACGCGCTTATGAAAACACTTCAGAGAGACTGCCGG
GTGCTAGAAAGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCA
TTTCCTGGACCATTCCCTCAAAGAGAAAGAGGAGGAGACCCCACAAAATG
TGCCTGATGCTAAGCGGACTGGGATGCAGAAGGTTCTCTACTCAACAGCC
ATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGGATAATCACAGA
GAACCCAGACACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAAA
AACTACTTTTATTTACGGGCATTATTGACATTCTGCAATCATATAGGTTA
ATGAAGAAGTTAGAACATTCCTGGAAAGCTCTTGTTTATGATGGGGACAC
TGTTTCTGTTCATAGACCAAGCTTTTATGCAGACAGATTTCTTAAGTTCA
TGAATTCCAGAGTTTTCAAGAAAATTCAAGCTTTGAAGGCTTCACCGTCT
AAGAAACGGTGCAATTCAATCGCCGCCCTAAAGGCCACTTCACAGGAGAT
TGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGGGATTTGCTGA
CTGAAGGACAAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGA
CACAGGCCAGACCTGGTCCCTAGCACTCCATCACTGTTTGAAGCTGCTTC
CTTGGCAACCACAATTTCATCTTCTTCCTTATACGTCAATGAGCACTATC
CACACGACAGGCCTACACTCTATTCAAACAGGTTCAAGATGGCAACATCA
GAGCATTAAGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMSSAAENG
EAAPGKQNEEKTYKKTQVKNQILSRLPKIPCTFIQAWTNTEMITLLACYY
FRSVSTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPSE
GSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELS
NPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLP
KFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKER
EKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYS
LLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSG
DGIITENPDTMGGIPAKSHRGEKLLLFTGIIDILQSYRLMKKLEHSWKAL
VYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAALK
ATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPS
LFEAASLATTISSSSLYVNEHYPHDRPTLYSNRFKMATSEH

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 90256 Daltons