cDNA Clone PIP5K2B

• Plasmid: pGEX6P-1-PIP5K2B
• Parent Plasmid: pGEX6P1
• Expressed: GST PIP5K2B
• Genbank: 1BO1_A
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGGCGCC
GCTCAGCGCCAGCAAGACCAAGACCAAGAAGAAGCATTTCGTGTGCCAGA
AAGTGAAGCTATTCCGGGCCAGCGAGCCGATCCTCAGCGTCCTGATGTGG
GGGGTGAACCACACGATCAATGAGCTGAGCAATGTTCCTGTTCCTGTCAT
GCTAATGCCAGATGACTTCAAAGCCTACAGCAAGATCAAGGTGGACAATC
ATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTAAGTTTAAGGAGTAT
TGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGGAATTGATGATCA
GGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGACAGCC
AGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGACCGGCGCTTTGTC
ATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATCTTAAA
GAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTTGCCAC
AGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTGGAAACCTACATG
GTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGTGCATCGCAAGTA
TGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCGACAAGGAGAAGG
CCAAGGACTTGCCAACATTCAAAGACAATGACTTCCTCAATGAAGGGCAG
AAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTCCTGGAGAAACTGAA
GCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAGCCTGC
TGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGGAGGTG
GAGGAGCGGGCAGAGGACGAGGAGTGTGAGAATGATGGGGTGGGTGGCAA
CCTACTCTGCTCCTATGGCACACCTCCGGACAGCCCTGGCAACCTCCTCA
GCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCCTCTGTTGACGTC
TATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAGGAGGTGTATTTTAT
GGCCATCATTGATATCCTCACGCCATACGATACCAAGAAGAAAGCTGCAC
ATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGACTGTG
AACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAACATCCT
GACGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSSNCTSTTAVAVAPLSAS
KTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPD
DFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQN
SVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQ
FIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKG
STVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVE
FLAQLKIMDYSLLVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCS
YGTPPDSPGNLLSFPRFFGPGEFDPSVDVYAMKSHESSPKKEVYFMAIID
ILTPYDTKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32570
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 74200 Daltons