cDNA Clone UBE2T

• Plasmid: pcDNA3.1 Hygro UBE2T FLAG
• Parent Plasmid: pcDNA3.1 hygro C-FLAG
• Expressed: UBE2T FLAG
• Genbank: NP_054895.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCAGAGAGCTTCACGTCTGAAGAGAGAGCTGCACATGTTAGC
CACAGAGCCACCCCCAGGCATCACATGTTGGCAAGATAAAGACCAAATGG
ATGACCTGCGAGCTCAAATATTAGGTGGAGCCAACACACCTTATGAGAAA
GGTGTTTTTAAGCTAGAAGTTATCATTCCTGAGAGGTACCCATTTGAACC
TCCTCAGATCCGATTTCTCACTCCAATTTATCATCCAAACATTGATTCTG
CTGGAAGGATTTGTCTGGATGTTCTCAAATTGCCACCAAAAGGTGCTTGG
AGACCATCCCTCAACATCGCAACTGTGTTGACCTCTATTCAGCTGCTCAT
GTCAGAACCCAACCCTGATGACCCGCTCATGGCTGACATATCCTCAGAAT
TTAAATATAATAAGCCAGCCTTCCTCAAGAATGCCAGACAGTGGACAGAG
AAGCATGCAAGACAGAAACAAAAGGCTGATGAGGAAGAGATGCTTGATAA
TCTACCAGAGGCTGGTGACTCCAGAGTACACAACTCAACACAGAAAAGGA
AGGCCAGTCAGCTAGTAGGCATAGAAAAGAAATTTCATCCTGATGTTCTC
GAG

Amino Acid Sequence

MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGV
FKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRP
SLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKH
ARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDVLEG
AAAGDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32561
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter FLAG tag for mammalian expression with hygromycin selection; ; Estimated MW of expressed protein : 24070 Da