cDNA Clone MAP3K13

• Plasmid: pCMV-FLAG-2 MAP3K13
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG MAP3K13
• Genbank: NM_004721.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCAACTTTCAGGAGCACCTGAGCTGCTCCTCTTCTCC
ACACTTACCCTTCAGTGAAAGCAAAACCTTCAATGGACTACAAGATGAGC
TCACAGCTATGGGGAACCACCCTTCTCCCAAGCTGCTCGAGGACCAGCAG
GAAAAGGGGATGGTACGAACAGAGCTAATCGAGAGCGTGCACAGCCCCGT
CACCACAACAGTGTTGACGAGCGTAAGTGAGGACTCCAGGGACCAGTTTG
AGAACAGCGTTCTTCAGCTAAGGGAACACGATGAATCAGAGACGGCGGTG
TCTCAGGGGAACAGCAACACGGTGGACGGAGAGAGCACAAGCGGAACTGA
AGACATAAAGATTCAGTTCAGCAGGTCAGGCAGTGGCAGTGGTGGGTTTC
TTGAAGGACTATTTGGATGCTTAAGGCCTGTATGGAATATCATTGGGAAG
GCATATTCCACTGATTACAAATTGCAGCAGCAAGATACTTGGGAAGTGCC
ATTTGAGGAGATCTCAGAGCTGCAGTGGCTGGGTAGTGGAGCCCAAGGAG
CGGTCTTCTTGGGCAAGTTCCGGGCGGAAGAGGTGGCCATCAAGAAAGTG
AGAGAACAGAATGAGACGGATATCAAGCATTTGAGGAAGTTGAAGCACCC
TAACATCATCGCATTCAAGGGTGTTTGTACTCAGGCCCCATGTTATTGTA
TTATCATGGAATACTGTGCCCATGGACAACTCTACGAGGTCTTACGAGCT
GGCAGGAAGATCACACCTCGATTGCTAGTAGACTGGTCCACAGGAATTGC
AAGTGGAATGAATTATTTGCACCTCCATAAAATTATTCATCGTGATCTCA
AATCACCTAATGTTTTAGTGACCCACACAGATGCGGTAAAAATTTCAGAT
TTTGGTACATCTAAGGAACTCAGTGACAAAAGTACCAAGATGTCATTTGC
TGGCACGGTCGCATGGATGGCGCCAGAGGTGATACGGAATGAACCTGTCT
CTGAAAAAGTTGATATATGGTCTTTTGGAGTGGTGCTTTGGGAGCTGCTG
ACAGGAGAGATCCCTTACAAAGACGTAGATTCTTCAGCCATTATCTGGGG
TGTTGGAAGCAACAGCCTCCACCTTCCAGTTCCTTCCACTTGCCCTGATG
GATTCAAAATCCTTATGAAACAGACGTGGCAGAGTAAACCTCGAAACCGA
CCTTCTTTTCGGCAGACACTCATGCATTTAGACATTGCCTCTGCAGATGT
ACTTGCCACCCCACAAGAAACTTACTTCAAGTCTCAGGCTGAATGGAGAG
AAGAAGTGAAAAAACATTTTGAGAAGATCAAAAGTGAAGGAACTTGTATA
CACCGGTTAGATGAAGAACTGATTCGAAGGCGCAGAGAAGAGCTCAGGCA
TGCGCTGGATATTCGTGAACACTATGAGCGGAAGCTTGAGCGGGCGAATA
ATTTATACATGGAATTGAGTGCCATCATGCTGCAGCTAGAAATGCGGGAG
AAGGAGCTCATTAAGCGTGAGCAAGCAGTGGAAAAGAAGTATCCTGGGAC
CTACAAACGACACCCTGTTCGTCCTATCATCCATCCCAATGCCATGGAGA
AACTCATGAAAAGGAAAGGAGTGCCTCACAAATCTGGGATGCAGACCAAA
CGGCCAGACTTGTTGAGATCAGAAGGGATCCCCACCACAGAAGTGGCTCC
CACTGCATCCCCTTTGTCCGGAAGTCCCAAAATGTCCACTTCTAGCAGCA
AGAGCCGATATCGAAGCAAACCACGCCACCGCCGAGGGAATAGCAGAGGC
AGCCATAGTGACTTTGCCGCAATCTTGAAAAACCAGCCAGCCCAGGAAAA
TTCACCCCATCCCACTTACCTGCACCAAGCTCAATCCCAATACCCTTCTC
TTCATCACCATAATTCTCTGCAGCAGCAATACCAGCAGCCCCCTCCTGCC
ATGTCCCAGAGTCACCATCCCAGACTCAATATGCACGGACAGGACATAGC
AACCTGCGCCAACAACCTGAGGTATTTCGGCCCAGCAGCAGCCCTGCGGA
GCCCACTCAGCAACCATGCTCAGAGACAGCTGCCCGGCTCGAGCCCTGAC
CTCATCTCCACAGCCATGGCTGCAGACTGCTGGAGAAGTTCTGAGCCTGA
CAAGGGCCAAGCTGGTCCCTGGGGCTGTTGCCAGGCTGACGCTTATGACC
CCTGCCTTCAGTGCAGGCCAGAACAGTATGGGTCCTTAGACATACCCTCT
GCTGAGCCAGTGGGGAGGAGCCCTGACCTTTCCAAGTCACCAGCACATAA
TCCTCTCTTGGAAAACGCCCAGAGTTCTGAGAAAACGGAAGAAAATGAAT
TCAGCGGCTGTAGGTCTGAGTCATCCCTCGGCACCTCTCATCTCGGCACC
CCTCCAGCGCTACCTCGAAAAACAAGGCCTCTGCAGAAGAGTGGAGATGA
CTCCTCAGAAGAGGAAGAAGGGGAAGTAGATAGTGAAGTTGAATTTCCAC
GAAGACAGAGGCCCCATCGCTGTATCAGCAGCTGCCAGTCATATTCAACC
TTTAGCTCTGAGAATTTCTCTGTGTCTGATGGAGAAGAGGGAAATACCAG
TGACCACTCAAACAGTCCTGATGAGTTAGCTGATAAACTTGAAGACCGCT
TGGCAGAGAAGCTAGACGACCTGCTGTCCCAGACGCCAGAGATTCCCATT
GACATATCCTCACACTCGGATGGGCTCTCTGACAAGGAGTGTGCCGTGCG
CCGTGTGAAGACTCAGATGTCTCTGGGCAAGCTGTGTGTGGAGGAACGTG
GCTATGAGAACCCCATGCAGTTTGAAGAATCGGACTGTGACTCTTCAGAT
GGGGAGTGTTCTGATGCCACAGTTAGGACCAATAAACACTACAGCTCTGC
TACCTGGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMANFQEHLSCSSSPHLPFSESKTFNGLQ
DELTAMGNHPSPKLLEDQQEKGMVRTELIESVHSPVTTTVLTSVSEDSRD
QFENSVLQLREHDESETAVSQGNSNTVDGESTSGTEDIKIQFSRSGSGSG
GFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDTWEVPFEEISELQWLGSGA
QGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPNIIAFKGVCTQAPC
YCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKIIHR
DLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNE
PVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTC
PDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLATPQETYFKSQAE
WREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLER
ANNLYMELSAIMLQLEMREKELIKREQAVEKKYPGTYKRHPVRPIIHPNA
MEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTS
SSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQY
PSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAA
LRSPLSNHAQRQLPGSSPDLISTAMAADCWRSSEPDKGQAGPWGCCQADA
YDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNPLLENAQSSEKTEE
NEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDSSEEEEGEVDSEVE
FPRRQRPHRCISSCQSYSTFSSENFSVSDGEEGNTSDHSNSPDELADKLE
DRLAEKLDDLLSQTPEIPIDISSHSDGLSDKECAVRRVKTQMSLGKLCVE
ERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32354
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; Estimated MW of expressed protein : 110568 Daltons