cDNA Clone ISG15

• Plasmid: pGEX6P-1-ISG15 1-157
• Parent Plasmid: pGEX6P1
• Expressed: GST ISG15 1-157
• Genbank: NP_005092
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATT
CCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGA
TCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCAC
CCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCT
GGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTC
TGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTG
CGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGA
GGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGG
AGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTG
TTCATGAATCTGCGCCTGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGC
TAAACAGAGCCTGGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGWDLTVKMLAGNEFQVSL
SSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGS
TVLLVVDKCDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQD
DLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-ISG15 1-157313.49 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU32336
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; Estimated MW of expressed protein : 43996 Daltons
• Low Temp: No