cDNA Clone PIP5K1A

• Plasmid: pET156P PIP5K1A
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His PIP5K1A
• Genbank: NP_003548.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGTCGGCCTCCTCCGGGCCGTCGTCTTCGGTCGGTTTTTC
ATCCTTTGATCCCGCGGTCCCTTCCTGTACCTTGTCCTCAGCATCTGGAA
TCAAGAGACCCATGGCATCTGAGGTGCCTTATGCCTCTGGCATGCCCATC
AAGAAAATAGGCCATAGAAGTGTTGATTCCTCAGGAGAGACAACATATAA
AAAGACAACCTCATCAGCCTTGAAAGGTGCCATCCAGTTAGGCATTACCC
ACACTGTGGGGAGCCTGAGTACCAAACCAGAGCGTGATGTCCTCATGCAA
GATTTCTACGTGGTTGAGAGTATCTTCTTTCCCAGTGAAGGGAGCAACCT
GACCCCTGCTCATCACTACAATGACTTTCGTTTCAAGACCTATGCACCTG
TTGCCTTCCGCTACTTCCGGGAGCTATTTGGTATCCGGCCCGATGATTAC
TTGTATTCCCTCTGCAGTGAGCCGCTGATTGAACTCTGTAGCTCTGGAGC
TAGTGGTTCCCTATTCTATGTGTCCAGCGACGATGAGTTCATTATTAAGA
CAGTCCAACATAAAGAGGCGGAATTTCTGCAGAAGCTGCTTCCAGGATAC
TACATGAACCTCAACCAGAACCCTCGGACTTTGCTGCCTAAATTCTATGG
ACTGTACTGTGTGCAGGCAGGTGGCAAGAACATTCGGATTGTGGTGATGA
ACAATCTTTTACCAAGATCGGTAAAAATGCATATCAAATATGACCTCAAA
GGCTCAACCTACAAACGGCGGGCTTCCCAGAAAGAGCGAGAGAAGCCTCT
TCCCACATTTAAAGACCTAGACTTCTTACAAGACATCCCTGATGGTCTTT
TTTTGGATGCTGACATGTACAACGCTCTCTGTAAGACCCTGCAGCGTGAC
TGTTTGGTGCTGCAGAGCTTCAAGATAATGGATTACAGCCTCTTGATGTC
AATCCATAATATAGATCATGCACAACGAGAGCCCTTAAGCAGTGAAACAC
AGTACTCAGTTGATACTCGAAGACCGGCCCCCCAAAAGGCTCTGTATTCC
ACAGCCATGGAATCCATCCAGGGAGAGGCTCGACGGGGTGGTACCATGGA
GACTGATGACCATATGGGTGGCATCCCTGCCCGGAATAGTAAAGGGGAAA
GGCTTCTGCTTTATATTGGCATCATTGACATTCTACAGTCTTACAGGTTT
GTTAAGAAGTTGGAGCACTCCTGGAAAGCCCTGGTACATGACGGAGACAC
TGTCTCAGTGCATCGCCCAGGCTTCTACGCTGAACGGTTCCAGCGCTTCA
TGTGCAACACAGTATTTAAGAAGATTCCCTTGAAGCCTTCTCCTTCCAAA
AAGTTTCGGTCTGGCTCATCTTTCTCTCGGCGAGCAGGCTCCAGTGGCAA
CTCCTGCATTACTTACCAGCCATCGGTCTCTGGGGAACACAAGGCACAAG
TGACAACAAAGGCAGAAGTGGAGCCAGGCGTTCACCTTGGTCGTCCTGAT
GTTTTACCTCAGACTCCACCTTTGGAGGAAATCAGTGAGGGCTCGCCTAT
TCCTGACCCCAGTTTCTCACCTCTAGTTGGAGAGACTTTGCAAATGCTAA
CTACAAGTACAACCTTGGAAAAGCTTGAAGTTGCAGAGTCAGAGTTCACC
CATTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMASASSGPSSSVGFSSFDPAVPSCTLS
SASGIKRPMASEVPYASGMPIKKIGHRSVDSSGETTYKKTTSSALKGAIQ
LGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFK
TYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDE
FIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIR
IVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDI
PDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPL
SSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARN
SKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAER
FQRFMCNTVFKKIPLKPSPSKKFRSGSSFSRRAGSSGNSCITYQPSVSGE
HKAQVTTKAEVEPGVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGET
LQMLTTSTTLEKLEVAESEFTH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32319
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; Estimated MW of expressed protein : 63590 Daltons