cDNA Clone ETNK2

• Plasmid: pGEX6P-1-ETNK2
• Parent Plasmid: pGEX6P1
• Expressed: GST ETNK2
• Genbank: NP_060678
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTGTGCCCCCTTCGGCCCCTCAGCCGCGCGCGTCCTTTCA
CCTGAGGAGGCACACGCCTTGCCCGCAGTGCTCATGGGGCATGGAGGAGA
AGGCGGCGGCCAGCGCCAGCTGCCGGGAGCCGCCGGGCCCCCCGAGGGCC
GCCGCCGTCGCGTACTTCGGCATTTCCGTGGACCCGGACGACATCCTTCC
CGGGGCCCTGCGCCTCATCCAGGAGCTGCGGCCGCATTGGAAACCCGAGC
AAGTTCGGACCAAGCGCTTCACGGATGGCATCACCAACAAGCTGGTGGCC
TGCTATGTGGAGGAGGACATGCAGGACTGCGTGCTGGTCCGGGTGTATGG
GGAGCGGACGGAGCTGCTGGTGGACCGGGAGAATGAGGTCAGAAACTTCC
AGCTGCTGCGAGCACACAGCTGTGCCCCCAAACTCTACTGCACCTTCCAG
AATGGGCTGTGCTATGAGTACATGCAGGGTGTGGCCCTGGAGCCTGAGCA
CATCCGTGAGCCCCGGCTTTTCAGGTTAATCGCCTTAGAAATGGCAAAGA
TTCATACTATCCACGCCAACGGCAGCCTGCCCAAGCCCATCCTCTGGCAC
AAGATGCACAATTATTTCACGCTTGTGAAGAACGAGATCAACCCCAGCCT
TTCTGCAGATGTCCCTAAGGTAGAGGTGTTGGAACGGGAGCTGGCCTGGC
TGAAGGAGCATCTGTCCCAGCTGGAGTCCCCTGTGGTGTTTTGTCACAAT
GACCTGCTCTGCAAGAATATCATCTATGACAGCATCAAAGGTCACGTGCG
GTTCATTGACTATGAATATGCTGGCTACAACTACCAAGCTTTTGACATTG
GCAACCATTTCAATGAGTTTGCAGGCGTGAATGAGGTGGATTACTGCCTG
TACCCGGCGCGGGAGACCCAGCTGCAGTGGCTGCACTACTACCTGCAGGC
ACAAAAGGGGATGGCCGTGACCCCCAGGGAGGTGCAAAGGCTCTACGTGC
AAGTCAACAAGTTTGCCCTGGCGTCTCACTTCTTCTGGGCTCTCTGGGCC
CTCATCCAGAACCAGTACTCCACCATCGACTTTGATTTCCTCAGGTACGC
AGTGATCCGATTCAACCAGTACTTCAAGGTGAAGCCTCAAGCGTCAGCCT
TGGAGATGCCAAAGTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAVPPSAPQPRASFHLRRH
TPCPQCSWGMEEKAAASASCREPPGPPRAAAVAYFGISVDPDDILPGALR
LIQELRPHWKPEQVRTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTE
LLVDRENEVRNFQLLRAHSCAPKLYCTFQNGLCYEYMQGVALEPEHIREP
RLFRLIALEMAKIHTIHANGSLPKPILWHKMHNYFTLVKNEINPSLSADV
PKVEVLERELAWLKEHLSQLESPVVFCHNDLLCKNIIYDSIKGHVRFIDY
EYAGYNYQAFDIGNHFNEFAGVNEVDYCLYPARETQLQWLHYYLQAQKGM
AVTPREVQRLYVQVNKFALASHFFWALWALIQNQYSTIDFDFLRYAVIRF
NQYFKVKPQASALEMPK

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32284
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 71603 Daltons