cDNA Clone NEDD4

• Plasmid: pCMV-FLAG-1 NEDD4
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG NEDD4
• Genbank: NM_006154.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGA
SDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLF
EVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSR
VKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQE
PSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQ
AQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAFPSPPPSS
NLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPT
LPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQ
LTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFF
IDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRI
FYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDI
PNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVA
REWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIG
RVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWIL
ENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQW
RFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWR
EHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFA
ELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMA
IENTQGFDGVD

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32250
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; ; Estimated MW of expressed protein : 105429 Daltons