cDNA Clone HOIL

• Synonyms: XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3; RBCK1
• Plasmid: pET15b 6His HOIL
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C HOIL
• Genbank: NM_031229.4 NP_112506.2
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAG
CCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTG
CCATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCT
GAGGTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGC
TCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAG
TGGCGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTC
TTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCT
GCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGC
TGTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGG
CAGCTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCC
GCGGGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAAC
CCGGACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGG
CAGTGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGA
GATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACC
AGCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTG
CGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCA
GCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCG
AGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTG
CGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCG
CAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACT
CGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCT
GAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCG
CAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCT
TCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTC
AACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTA
TCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGA
CGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGC
CCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCG
CTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCT
GGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTA
AATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDEKTKKAEEMALSL
TRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLWVSVEDAQ
MHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLH
SHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPR
GPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKPTRPGCEM
CCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQH
VQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRN
SQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRS
AFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQ
EDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRC
TVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6His HOIL99.1 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU32155
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed : 61184 Daltons
• Low Temp: No