cDNA Clone PP4R3A

• Plasmid: pFB6P-1 PP4R3A
• Parent Plasmid: pFastbac GST
• Expressed: GST PP4R3A
• Genbank: AAH72409
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGA
GGACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTCTGGCTACG
TGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGACGGT
TCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACA
ACAGGACACTCTGATTGTGTGGTCTGAAGCAGAAAATTATGACTTGGCCC
TTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAGAAAATATGT
CAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGA
TGAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAAT
TGCCATCTTGTGAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCA
TCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCACTAGA
AAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAG
ATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATTATCAAAGGC
ATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGA
ATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCAC
AACCACGAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAA
GTGATTCCCATATCAGATCCTGAGCTGAAACAAAAAATTCATCAGACATA
CAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTCTTTG
AAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTA
GAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTT
TGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGG
TTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAA
AACAGAGATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGC
TTTAGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAGTGCTGCTA
CTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAG
TTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAAC
AGAGCAAAAAATAACCAGCAAGGATATTTTGCTCATCAACCTCATTATAG
AACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTT
ATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGC
CAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTA
TGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCT
AGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATT
GTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTA
TTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCAT
GCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATT
AAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAAC
CAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAAC
TCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATC
ATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGATGTAG
ATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGA
GAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAA
TCACAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGT
GGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCT
CCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAA
ATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTC
TGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTG
TCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCT
GCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTG
GATCCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGA
GGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGA
GGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTG
ATTCATAAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMTDTRRRVKVYTLNED
RQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQ
DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDE
SEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALEN
EGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEEC
IMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYR
VQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFA
QLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPAL
EVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTE
QKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATAN
KTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELL
TFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLK
DEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSL
TAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNH
RYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKF
MERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLP
GSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDED
EDKEDTLPLSKKAKFDS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32125
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expresion; ; Estimated MW of expressed protein : 122563 Daltons