cDNA Clone IRAK1 (mouse)

• Plasmid: pGEX6P-1-IRAK1 (mouse)
• Parent Plasmid: pGEX6P1
• Expressed: GST IRAK1 (mouse)
• Genbank: AAH04778
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGCCTT
GATCGTGCGCGACCAGACAGAGCTGCGGCTGTGCGAGCGCTCCGAGCAGC
GCACAGCCAGTGTTCTGTGGCCCTGGATCAACCGCAACGCGCGCGTAGCT
GACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGCGTGCGAGGGACAT
CATCACAGCCTGGCACCCTCCTGCCCCCGTTGTGCCCCCAAGCACCGCTG
CCCCAAGGCCCAGCAGCATCTCTGCAGGCTCTGAGGCCGGGGACTGGAGC
CCCCGGAAATTGCAGTCCTCTGCCTCCACCTTCCTCTCCCCAGCTTTTCC
AGGCTCCCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCCCTG
TTTCCCTCGGGCCACCACTACCATCTTCAGCCCCTTCCTCCACCAAGCAG
TCAAGCCCAGAGAGCCCAGTGTCTGGCCTCCAAAGAGCCCATCCCTCCCC
GTTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACCTGCAACTTCTCTG
AAGAACTCAGGATTGGAGAGGGTGGTTTTGGATGTGTGTACCGAGCAGTC
ATGAGAAATACTACATATGCTGTGAAGAGACTGAAGGAGGAAGCTGACCT
AGAGTGGACTATGGTGAAACAGAGCTTCTTAACAGAGGTGGAACAGCTAT
CAAGGTTTCGTCACCCAAATATCGTAGACTTTGCTGGCTACTGTGCAGAG
AGTGGCTTATACTGCCTTGTTTATGGCTTCTTGCCCAATGGCTCCTTGGA
GGATCAGCTCCACCTTCAGACCCAGGCCTGCTCCCCACTTTCCTGGCCTC
AACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCAGTTTTTACAT
CAGGATAGCCCCAGCCTTATCCATGGAGACATCAAGAGTTCTAACGTGCT
TCTGGATGAGAGACTGATGCCCAAGCTGGGAGACTTTGGCCTGGCTCGTT
TCAGCCGCTTTGCGGGGGCCAAAGCAAGCCAGAGCAGTACTGTGGCCCGG
ACTTCCACAGTTCGAGGTACCCTCGCCTACTTGCCTGAGGAGTACATCAA
GACAGGCCGACTGGCTGTGGACACCGATACCTTCAGCTTTGGGGTGGTAA
TACTGGAGACCCTTGCTGGTCAGAGGGCTGTGAGGACACAAGGTGCAAAG
ACCAAGTATTTGAAAGACCTGATTGAAGATGAGGCTGAAGAGGCTGGAGT
GACCCTGAAAAGCACCCAGCCTACTCTGTGGGTGGGTGTAGCCACGGATG
CTTGGGCTGCTCCAATTGCTGCCCAGATCTATAAGAAGCACCTGGACTCC
AGACCTGGGCCCTGCCCACCCCAGTTGGGCCTGGCCCTGGCTCAACTAGC
TTGCTGCTGCATGCACCGTCGGGCCAAGAAGAGGCCCCCCATGACCCAGA
GGCAAAACTCCCAACATTCCTTGCTTGCCCTTTGGCTCTATTTGGGGAGG
TGCTCTGTGGAACTAAGTGGTAAAGGGCTGCTGCTTTGCTGGATGAGCCT
CAGATCCCAGGAACAGGTATACAAGAGACTAGAAGGGCTTCAGGCAGGGC
CTCCCTGGGAGCTAGAGGTTGCCGGCCATGGCTCCCCTTCCCCACAGGAG
AACTCCTACATGTCTACCACTGGCAGTGCCCAGAGTGGGGATGAACCATG
GCAGCCTCTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAAC
TCCAGAGAAGTCCCAACCAGCCAGTGGAAAGTGATGAGAGTGTTCCCGGC
CTCTCTGCTACCCTGCATTCCTGGCACTTGACTCCAGGTTCCCACCCAAG
CCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACCAGAG
AATCAAGTGTGAGGAGTAGCCCAGGCTTCCAGCCTACAACCATGGAAGGC
TCACCCACGGGCAGCTCATCCCTGCTGTCATCAGAACCACCACAGATCAT
CATCAACCCAGCCCGACAGAAGATGGTACAAAAGCTGGCTCTTTATGAAG
AAGGGGTCTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGC
TTGGATTTAGAACCTGAAAAGAGCCAGGGACCTGAAGAAAGTGATGAATT
CCAGAGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDALEPADWCQFAALIVRD
QTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAW
HPPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSASTFLSPAFPGSQT
HSESELLQVPLPVSLGPPLPSSAPSSTKQSSPESPVSGLQRAHPSPFCWP
FCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTM
VKQSFLTEVEQLSRFRHPNIVDFAGYCAESGLYCLVYGFLPNGSLEDQLH
LQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDER
LMPKLGDFGLARFSRFAGAKASQSSTVARTSTVRGTLAYLPEEYIKTGRL
AVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEAGVTLKS
TQPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCM
HRRAKKRPPMTQRQNSQHSLLALWLYLGRCSVELSGKGLLLCWMSLRSQE
QVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQSGDEPWQPLV
VTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASF
REASCTQGGTTRESSVRSSPGFQPTTMEGSPTGSSSLLSSEPPQIIINPA
RQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKSQGPEESDEFQS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32043
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 105105 Daltons