cDNA Clone PCTK1

• Plasmid: pGEX6P-2-PCTK1 K194A
• Parent Plasmid: pGEX6P2
• Expressed: GST PCTK1 K194A
• Genbank: NM_006201
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGAC
ACTCCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGA
TAGGCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGCC
CCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAG
CTCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGG
AGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTG
AGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACAT
CAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACC
TGGAGAAGCTGACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGC
CGCCTCCGTCGTGTCAGCCTATCTGAGATTGGCTTTGGGAAACTGGAGAC
CTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACA
AAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCGCGGAGATCAGA
CTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCT
ACTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCC
ACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTG
AAGCAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAACGTGAA
ACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAGA
AGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGG
GGAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCC
AACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCCCCCTG
ACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGT
GTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGG
CTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCC
CAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACA
TACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCG
ACTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGG
GTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCTTCCTC
AGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGC
ACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGA
TGCCTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
GCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDRMKKIK
RQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRS
ARGPLSSAPEIVHEDLKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRK
ISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIG
FGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALAEIRLEHEEGAPCTA
IREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNII
NMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLA
RAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATG
RPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEAL
LSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPD
TTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32029
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; ; Estimated MW of expressed protein : 83459 Daltons