cDNA Clone NSF

• Plasmid: pEBG6P-NSF
• Parent Plasmid: pGEX6P2
• Expressed: GST NSF
• Genbank: NM_006178.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGA
TGAATTATCTTTAACCAATTGTGCAGTTGTGAATGAAAAGGATTTCCAGT
CTGGCCAGCATGTGATTGTGAGGACCTCTCCCAATCACAGGTACACATTT
ACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATTGCATTCAGTTT
ACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAGAAGTCT
CCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATC
GAGATTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACAC
CGACAAGATGGCAGCAGAATTTATTCAGCAATTCAACAACCAGGCCTTCT
CAGTGGGACAACAGCTTGTCTTTAGCTTCAATGAAAAGCTTTTTGGCTTA
CTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTGAAGGGAGAGCC
TGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAACA
GTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGC
AAAGCTAAAACCAAGGAAAATCGCCAATCAATTATCAATCCTGACTGGAA
CTTTGAAAAAATGGGAATAGGAGGTCTAGACAAGGAATTTTCAGATATTT
TCCGACGAGCATTTGCTTCCCGAGTATTTCCTCCAGAGATTGTGGAGCAG
ATGGGTTGTAAACATGTTAAAGGCATCCTGTTATATGGACCCCCAGGTTG
TGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGAG
AGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAA
TCAGAGGCTAACATTCGCAAACTTTTTGCTGATGCTGAAGAGGAGCAAAG
GAGGCTTGGTGCTAACAGTGGTTTGCACATCATCATCTTTGATGAAATTG
ATGCCATCTGCAAGCAGAGAGGGAGCATGGCTGGTAGCACGGGAGTTCAT
GACACTGTTGTCAACCAGTTGCTGTCCAAAATTGATGGCGTGGAGCAGCT
AAACAACATCCTAGTCATTGGAATGACCAATAGACCAGATCTGATAGATG
AGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTTG
CCAGATGAGAAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAAT
GAGAGGGCATCAGTTACTCTCTGCTGATGTAGACATTAAAGAACTGGCCG
TGGAGACCAAGAATTTCAGTGGTGCTGAATTGGAGGGTCTAGTGCGAGCA
GCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGTACTAAAGTGGA
AGTGGACATGGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTCC
TTGCTTCTTTGGAGAATGATATCAAACCAGCCTTTGGCACAAACCAAGAA
GATTATGCAAGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAGT
TACTCGAGTTCTAGATGATGGGGAGCTGCTGGTGCAGCAGACTAAGAACA
GTGACCGCACACCATTGGTCAGCGTGCTTCTGGAAGGCCCTCCTCACAGT
GGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCCAACTTCCCTTT
CATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCCA
AATGTCAGGCCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTC
AGTTGTGTGGTTGTGGATGACATTGAGAGATTGCTTGATTACGTCCCTAT
TGGCCCTCGATTTTCAAATCTTGTATTACAGGCTCTTCTCGTTTTACTGA
AAAAGGCACCTCCTCAGGGCCGCAAGCTTCTTATCATTGGGACCACTAGC
CGCAAAGATGTCCTTCAGGAGATGGAAATGCTTAACGCTTTCAGCACCAC
CATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTTGGAAGCTTTGG
AGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAA
GTCAAAGGGAAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGAT
CGAGATGTCCCTACAGATGGATCCTGAATACCGTGTGAGAAAATTCTTGG
CCCTCTTAAGAGAAGAAGGAGCTAGCCCCCTTGATTTTGATTGAGCGGCC
GC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAGR
SMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPS
VVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQK
KSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEA
MDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKEN
RQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVK
GILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRK
LFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQL
LSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRL
QILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMN
RHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIM
NGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAA
KIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDD
IERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQE
MEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVW
IGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32028
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 110796 Daltons