cDNA Clone NSF

• Plasmid: pMalpP NSF
• Parent Plasmid: pMAL6P
• Expressed: MBP NSF
• Genbank: NM_006178.2
• Tag: MBP
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGA
TGAATTATCTTTAACCAATTGTGCAGTTGTGAATGAAAAGGATTTCCAGT
CTGGCCAGCATGTGATTGTGAGGACCTCTCCCAATCACAGGTACACATTT
ACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATTGCATTCAGTTT
ACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAGAAGTCT
CCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATC
GAGATTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACAC
CGACAAGATGGCAGCAGAATTTATTCAGCAATTCAACAACCAGGCCTTCT
CAGTGGGACAACAGCTTGTCTTTAGCTTCAATGAAAAGCTTTTTGGCTTA
CTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTGAAGGGAGAGCC
TGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAACA
GTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGC
AAAGCTAAAACCAAGGAAAATCGCCAATCAATTATCAATCCTGACTGGAA
CTTTGAAAAAATGGGAATAGGAGGTCTAGACAAGGAATTTTCAGATATTT
TCCGACGAGCATTTGCTTCCCGAGTATTTCCTCCAGAGATTGTGGAGCAG
ATGGGTTGTAAACATGTTAAAGGCATCCTGTTATATGGACCCCCAGGTTG
TGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGAG
AGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAA
TCAGAGGCTAACATTCGCAAACTTTTTGCTGATGCTGAAGAGGAGCAAAG
GAGGCTTGGTGCTAACAGTGGTTTGCACATCATCATCTTTGATGAAATTG
ATGCCATCTGCAAGCAGAGAGGGAGCATGGCTGGTAGCACGGGAGTTCAT
GACACTGTTGTCAACCAGTTGCTGTCCAAAATTGATGGCGTGGAGCAGCT
AAACAACATCCTAGTCATTGGAATGACCAATAGACCAGATCTGATAGATG
AGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTTG
CCAGATGAGAAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAAT
GAGAGGGCATCAGTTACTCTCTGCTGATGTAGACATTAAAGAACTGGCCG
TGGAGACCAAGAATTTCAGTGGTGCTGAATTGGAGGGTCTAGTGCGAGCA
GCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGTACTAAAGTGGA
AGTGGACATGGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTCC
TTGCTTCTTTGGAGAATGATATCAAACCAGCCTTTGGCACAAACCAAGAA
GATTATGCAAGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAGT
TACTCGAGTTCTAGATGATGGGGAGCTGCTGGTGCAGCAGACTAAGAACA
GTGACCGCACACCATTGGTCAGCGTGCTTCTGGAAGGCCCTCCTCACAGT
GGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCCAACTTCCCTTT
CATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCCA
AATGTCAGGCCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTC
AGTTGTGTGGTTGTGGATGACATTGAGAGATTGCTTGATTACGTCCCTAT
TGGCCCTCGATTTTCAAATCTTGTATTACAGGCTCTTCTCGTTTTACTGA
AAAAGGCACCTCCTCAGGGCCGCAAGCTTCTTATCATTGGGACCACTAGC
CGCAAAGATGTCCTTCAGGAGATGGAAATGCTTAACGCTTTCAGCACCAC
CATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTTGGAAGCTTTGG
AGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAA
GTCAAAGGGAAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGAT
CGAGATGTCCCTACAGATGGATCCTGAATACCGTGTGAGAAAATTCTTGG
CCCTCTTAAGAGAAGAAGGAGCTAGCCCCCTTGATTTTGATTGAGCGGCC
GC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSPEFPGVDSAAAMAGRSMQAARC
PTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIA
FSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNP
YDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILK
GEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINP
DWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGP
PGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEE
EQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGV
EQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHT
ARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKAST
KVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWG
DPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESN
FPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDY
VPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAF
STTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLL
MLIEMSLQMDPEYRVRKFLALLREEGASPLDFD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 130371 Daltons