cDNA Clone NSF

• Plasmid: pCMV-HA-2 NSF
• Parent Plasmid: pCMV5 HA2
• Expressed: HA NSF
• Genbank: NM_006178.2
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGA
TGAATTATCTTTAACCAATTGTGCAGTTGTGAATGAAAAGGATTTCCAGT
CTGGCCAGCATGTGATTGTGAGGACCTCTCCCAATCACAGGTACACATTT
ACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATTGCATTCAGTTT
ACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAGAAGTCT
CCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATC
GAGATTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACAC
CGACAAGATGGCAGCAGAATTTATTCAGCAATTCAACAACCAGGCCTTCT
CAGTGGGACAACAGCTTGTCTTTAGCTTCAATGAAAAGCTTTTTGGCTTA
CTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTGAAGGGAGAGCC
TGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAACA
GTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGC
AAAGCTAAAACCAAGGAAAATCGCCAATCAATTATCAATCCTGACTGGAA
CTTTGAAAAAATGGGAATAGGAGGTCTAGACAAGGAATTTTCAGATATTT
TCCGACGAGCATTTGCTTCCCGAGTATTTCCTCCAGAGATTGTGGAGCAG
ATGGGTTGTAAACATGTTAAAGGCATCCTGTTATATGGACCCCCAGGTTG
TGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGAG
AGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAA
TCAGAGGCTAACATTCGCAAACTTTTTGCTGATGCTGAAGAGGAGCAAAG
GAGGCTTGGTGCTAACAGTGGTTTGCACATCATCATCTTTGATGAAATTG
ATGCCATCTGCAAGCAGAGAGGGAGCATGGCTGGTAGCACGGGAGTTCAT
GACACTGTTGTCAACCAGTTGCTGTCCAAAATTGATGGCGTGGAGCAGCT
AAACAACATCCTAGTCATTGGAATGACCAATAGACCAGATCTGATAGATG
AGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTTG
CCAGATGAGAAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAAT
GAGAGGGCATCAGTTACTCTCTGCTGATGTAGACATTAAAGAACTGGCCG
TGGAGACCAAGAATTTCAGTGGTGCTGAATTGGAGGGTCTAGTGCGAGCA
GCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGTACTAAAGTGGA
AGTGGACATGGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTCC
TTGCTTCTTTGGAGAATGATATCAAACCAGCCTTTGGCACAAACCAAGAA
GATTATGCAAGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAGT
TACTCGAGTTCTAGATGATGGGGAGCTGCTGGTGCAGCAGACTAAGAACA
GTGACCGCACACCATTGGTCAGCGTGCTTCTGGAAGGCCCTCCTCACAGT
GGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCCAACTTCCCTTT
CATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCCA
AATGTCAGGCCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTC
AGTTGTGTGGTTGTGGATGACATTGAGAGATTGCTTGATTACGTCCCTAT
TGGCCCTCGATTTTCAAATCTTGTATTACAGGCTCTTCTCGTTTTACTGA
AAAAGGCACCTCCTCAGGGCCGCAAGCTTCTTATCATTGGGACCACTAGC
CGCAAAGATGTCCTTCAGGAGATGGAAATGCTTAACGCTTTCAGCACCAC
CATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTTGGAAGCTTTGG
AGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAA
GTCAAAGGGAAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGAT
CGAGATGTCCCTACAGATGGATCCTGAATACCGTGTGAGAAAATTCTTGG
CCCTCTTAAGAGAAGAAGGAGCTAGCCCCCTTGATTTTGATTGAGCGGCC
GC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAGRSMQAARCPTDELSLTNCAVVNEK
DFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQE
IEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNN
QAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLV
VGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEF
SDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKML
NAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIF
DEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPD
LIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIK
ELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTR
GDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQ
TKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFS
ETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALL
VLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLL
EALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVR
KFLALLREEGASPLDFD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expresion; ; Estimated MW of expressed protein : 84955 Daltons