cDNA Clone PPM1E

• Plasmid: pGEX6P-PPM1E 495-755
• Parent Plasmid: pGEX6P1
• Expressed: GST PPM1E 495-755
• Genbank: CAH18109.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGTAAATGTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCA
AGGAGGGCAAGAAGATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCA
AACGCCCTTGGCCTCAGCACCAGTGCTCAGCACCAGCCGACCTAGGCTAT
GATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTC
CCAAATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAG
AAGCAAGCAAACCTCACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTT
GGTCCTGGTGCACCAAAGAAAGCAAATCTTATTAATGAGTTAATGATGGA
GAAAAAATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGT
TTCCCACTGCTTTCAATTTGGGTTCAACAGGGGAGCAGATATACAGAATG
CAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATC
CCCGGGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAA
AGAAGTGGCACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCT
GCTCAAGAGCCTTCCCACAAAATAGGCACTAGCCTGTCCTCACTTACTGG
AAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGCAAAATA
GTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGAT
ATTCCATGCCCAGATCTTCCTTGGAGCTATAAAATAGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVNVSEESDWTENSFQGGQE
DGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINV
LEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSV
QSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNR
VSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKR
NRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15986
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 56341 Daltons