cDNA Clone UBE2NL

• Plasmid: pEBG6P-UBE2NL
• Parent Plasmid: pEBG6P
• Expressed: GST UBE2NL
• Genbank: NP_001013007
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTCCGAAGGGCGAATTCTGCAGATATCCATCA
CACTGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAELPHRIIKETQR
LLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYP
MAAPKVRFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLN
APNPDDPLANDVVEQWKTNEAQAIETARAWTRLYAMNSI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15983
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.; ; EStimated MW of expressed protein : 44635 Daltons