cDNA Clone IKB-epsilon

• Plasmid: pCMV-HA IKB-epsilon
• Parent Plasmid: pCMV5 HA1
• Expressed: HA IKB-epsilon
• Genbank: AF261937
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTCGGAGGCGCGGAAGGGGCCGGACGAGGCGGAGGAGAGCCAGTA
CGACTCTGGCATTGAGTCTCTGCGCTCTCTGCGCTCCCTACCCGAGTCCA
CCTCGGCTCCAGCCTCCGGGCCCTCGGACGGCAGCCCCCAGCCCTGCACC
CATCCTCCGGGACCCGTCAAGGAACCACAGGAGAAGGAAGACGCGGATGG
GGAGCGGGCTGATTCCACCTATGGCTCCTCCTCGCTCACCTACACCCTGT
CCTTGCTGGGGGGCCCCGAGGCTGAGGACCCGGCCCCACGCCTGCCACTC
CCCCACGTGGGGGCGCTGAGCCCTCAGCAGCTGGAAGCACTCACTTACAT
CTCCGAGGACGGAGACACGCTGGTCCACCTGGCAGTGATTCATGAGGCCC
CAGCGGTGCTGCTCTGTTGCCTGGCTTTGCTGCCCCAGGAGGTCCTGGAC
ATTCAAAATAACCTTTACCAGACAGCACTCCATCTGGCTGTACATCTGGA
CCAACCGGGCGCAGTTCGGGCACTGGTGCTGAAGGGGGCCAGCCGGGCAC
TACAGGACCGGCATGGTGACACAGCCCTTCATGTGGCCTGCCAGCGCCAG
CACTTGGCCTGTGCCCGCTGCCTGCTGGAAGGGCGGCCAGAGCCAGGCAG
AGGAACATCTCACTCTCTGGACCTCCAGCTGCAAAACTGGCAAGGTCTGG
CTTGTCTCCACATTGCCACCCTTCAGAAGAACCAACCACTCATGGAATTG
CTGCTTCGGAATGGAGCTGACATTGATGTGCAGGAGGGCACCAGTGGTAA
GACAGCGCTGCACCTGGCTGTGGAAACCCAAGAGCGGGGCCTGGTACAGT
TCCTGCTCCAGGCTGGTGCCCAGGTAGATGCCCGCATGCTGAACGGGTGC
ACACCCCTGCACCTGGCAGCTGGCCGGGGTCTCATGGGCATCTCATCCAC
TCTGTGCAAGGCGGGTGCTGACTCCCTGCTGCGGAATGTGGAGGATGAGA
CGCCCCAGGACCTGACTGAGGAATCCCTTGTCCTTTTGCCCTTTGATGAC
CTGAAGATCTCAGGGAAACTGCTGCTGTGTACCGACTGAgaattc

Amino Acid Sequence

MYPYDVPDYAGSSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASG
PSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTYTLSLLGGPE
AEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCC
LALLPQEVLDIQNNLYQTALHLAVHLDQPGAVRALVLKGASRALQDRHGD
TALHVACQRQHLACARCLLEGRPEPGRGTSHSLDLQLQNWQGLACLHIAT
LQKNQPLMELLLRNGADIDVQEGTSGKTALHLAVETQERGLVQFLLQAGA
QVDARMLNGCTPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQDLTE
ESLVLLPFDDLKISGKLLLCTD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15873
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 39745 Daltons