cDNA Clone GYS1

• Plasmid: pCMV5 GYS1 S8A
• Parent Plasmid: pCMV5
• Expressed: GYS1 S8A
• Genbank: NM_002103
• Species: Human

Sequence of Insert

GGTACCACGCGTATCGATGCCACCATGCCTTTAAACCGCACTTTGGCCAT
GTCCTCACTGCCAGGACTGGAGGACTGGGAGGATGAATTCGACCTGGAGA
ACGCAGTGCTCTTCGAAGTGGCCTGGGAGGTGGCTAACAAGGTGGGTGGC
ATCTACACGGTGCTGCAGACGAAGGCGAAGGTGACAGGGGACGAATGGGG
CGACAACTACTTCCTGGTGGGGCCGTACACGGAGCAGGGCGTGAGGACCC
AGGTGGAACTGCTGGAGGCCCCCACCCCGGCCCTGAAGAGGACACTGGAT
TCCATGAACAGCAAGGGCTGCAAGGTGTATTTCGGGCGCTGGCTGATCGA
GGGAGGCCCTCTGGTGGTGCTCCTGGACGTGGGTGCCTCAGCTTGGGCCC
TGGAGCGCTGGAAGGGAGAGCTCTGGGATACCTGCAACATCGGAGTGCCG
TGGTACGACCGCGAGGCCAACGACGCTGTCCTCTTTGGCTTTCTGACCAC
CTGGTTCCTGGGTGAGTTCCTGGCACAGAGTGAGGAGAAGCCACATGTGG
TTGCTCACTTCCATGAGTGGTTGGCAGGCGTTGGACTCTGCCTGTGTCGT
GCCCGGCGACTGCCTGTAGCAACCATCTTCACCACCCATGCCACGCTGCT
GGGGCGCTACCTGTGTGCCGGTGCCGTGGACTTCTACAACAACCTGGAGA
ACTTCAACGTGGACAAGGAAGCAGGGGAGAGGCAGATCTACCACCGATAC
TGCATGGAAAGGGCGGCAGCCCACTGCGCTCACGTCTTCACTACTGTGTC
CCAGATCACCGCCATCGAGGCACAGCACTTGCTCAAGAGGAAACCAGATA
TTGTGACCCCCAATGGGCTGAATGTGAAGAAGTTTTCTGCCATGCATGAG
TTCCAGAACCTCCATGCTCAGAGCAAGGCTCGAATCCAGGAGTTTGTGCG
GGGCCATTTTTATGGGCATCTGGACTTCAACTTGGACAAGACCTTATACT
TCTTTATCGCCGGCCGCTATGAGTTCTCCAACAAGGGTGCTGACGTCTTT
CTGGAGGCATTGGCTCGGCTCAACTATCTGCTCAGAGTGAACGGCAGCGA
GCAGACAGTGGTTGCCTTCTTCATCATGCCAGCGCGGACCAACAATTTCA
ACGTGGAAACCCTCAAAGGCCAAGCTGTGCGCAAACAGCTTTGGGACACG
GCCAACACGGTGAAGGAAAAGTTCGGGAGGAAGCTTTATGAATCCTTACT
GGTTGGGAGCCTTCCCGACATGAACAAGATGCTGGATAAGGAAGACTTCA
CTATGATGAAGAGAGCCATCTTTGCAACGCAGCGGCAGTCTTTCCCCCCT
GTGTGCACCCACAATATGCTGGATGACTCCTCAGACCCCATCCTGACCAC
CATCCGCCGAATCGGCCTCTTCAATAGCAGTGCCGACAGGGTGAAGGTGA
TTTTCCACCCGGAGTTCCTCTCCTCCACAAGCCCCCTGCTCCCTGTGGAC
TATGAGGAGTTTGTCCGTGGCTGTCACCTTGGAGTCTTCCCCTCCTACTA
TGAGCCTTGGGGCTACACACCGGCTGAGTGCACGGTTATGGGAATCCCCA
GTATCTCCACCAACCTCTCCGGCTTCGGCTGCTTCATGGAGGAACACATC
GCAGACCCCTCAGCTTACGGTATCTACATTCTTGACCGGCGGTTCCGCAG
CCTGGATGATTCCTGCTCGCAGCTCACCTCCTTCCTCTACAGTTTCTGTC
AGCAGAGCCGGCGGCAGCGTATCATCCAGCGGAACCGCACGGAGCGCCTC
TCCGACCTTCTGGACTGGAAATACCTAGGCCGGTACTATATGTCTGCGCG
CCACATGGCGCTGTCCAAGGCCTTTCCAGAGCACTTCACCTACGAGCCCA
ACGAGGCGGATGCGGCCCAGGGGTACCGCTACCCACGGCCAGCCTCGGTG
CCACCGTCGCCCTCGCTGTCACGACACTCCAGCCCGCACCAGAGTGAGGA
TGAGGAGGATCCCCGGAACGGGCCGCTGGAGGAAGACGGCGAGCGCTACG
ATGAGGACGAGGAGGCCGCCAAGGACCGGCGCAACATCCGTGCACCAGAG
TGGCCGCGCCGAGCGTCCTGCACCTCCTCCACCAGCGGCAGCAAGCGCAA
CTCTGTGGACACGGCCACCTCCAGCTCACTCAGCACCCCGAGCGAGCCCC
TCAGCCCCACCAGCTCCCTGGGCGAGGAGCGTAACTAAGTCGAC

Amino Acid Sequence

MPLNRTLAMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTK
AKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCK
VYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREAND
AVLFGFLTTWFLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVAT
IFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAH
CAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQS
KARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLN
YLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKF
GRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD
DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGC
HLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGI
YILDRRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKY
LGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSR
HSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCT
SSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15796
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express untagged human muscle glycogen synthase.; ; Estimated MW of expressed protein : 83766 Daltons.