cDNA Clone TRIM21

• Synonyms: RO52, RNF81, SSA1
• Plasmid: pGEX6P-2-TRIM21
• Parent Plasmid: pGEX6P2
• Expressed: GST TRIM21
• Genbank: NM_003141
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGT
CACATGCCCTATCTGCCTGGACCCCTTCGTGGAGCCTGTGAGCATCGAGT
GTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGGG
GGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCG
GCCCAATCGACAGCTAGCCAACATGGTGAACAACCTTAAAGAAATCAGCC
AGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAGTGCATGGAGAG
AGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATG
TGCCCAGTCTCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGG
CTGCACAGGAGTACCAGGAGAAGCTCCAGGTGGCATTAGGGGAACTGAGA
AGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGAG
AGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAG
AGTTTGTGCAGCAAAAAAACTTCCTGGTTGAAGAAGAACAGAGGCAGCTG
CAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATCCTGGGGGAGAA
AGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAG
AGCTAGATCGAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTG
ATAATTGTCCTGGAAAGGAGTGAGTCCTGGAACCTGAAGGACCTGGATAT
TACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAGA
TGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAAT
CCGTGGCTGATACTTTCAGAAGATCGGAGACAAGTGAGGCTTGGAGACAC
CCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAGTTATCCTATGG
TCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGAT
GTGACAGGAAAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCG
CAGGAAGGGGCACTTTTTGCTTAGTTCCAAGAGTGGCTTCTGGACAATTT
GGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCCC
CTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGA
GGCTGGCATGGTCTCCTTCTACAACATCACTGACCATGGCTCCCTCATCT
ACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGCCCTTCTTCAGT
CCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCC
ACTGAATATTGGATCACAAGGATCCACTGACTATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMASAARLT
MMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRF
LLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGK
ALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEV
EIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQL
RILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWN
LKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQ
VRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGV
CRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVG
IFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTA
PLTLCPLNIGSQGSTDY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15748
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 81970 Daltons