cDNA Clone TRIM21

• Synonyms: RO52, RNF81, SSA1
• Plasmid: pCMV-HA-2 TRIM21
• Parent Plasmid: pCMV5 HA2
• Expressed: HA TRIM21
• Genbank: NM_003141
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGT
CACATGCCCTATCTGCCTGGACCCCTTCGTGGAGCCTGTGAGCATCGAGT
GTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGGG
GGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCG
GCCCAATCGACAGCTAGCCAACATGGTGAACAACCTTAAAGAAATCAGCC
AGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAGTGCATGGAGAG
AGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATG
TGCCCAGTCTCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGG
CTGCACAGGAGTACCAGGAGAAGCTCCAGGTGGCATTAGGGGAACTGAGA
AGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGAG
AGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAG
AGTTTGTGCAGCAAAAAAACTTCCTGGTTGAAGAAGAACAGAGGCAGCTG
CAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATCCTGGGGGAGAA
AGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAG
AGCTAGATCGAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTG
ATAATTGTCCTGGAAAGGAGTGAGTCCTGGAACCTGAAGGACCTGGATAT
TACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAGA
TGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAAT
CCGTGGCTGATACTTTCAGAAGATCGGAGACAAGTGAGGCTTGGAGACAC
CCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAGTTATCCTATGG
TCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGAT
GTGACAGGAAAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCG
CAGGAAGGGGCACTTTTTGCTTAGTTCCAAGAGTGGCTTCTGGACAATTT
GGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCCC
CTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGA
GGCTGGCATGGTCTCCTTCTACAACATCACTGACCATGGCTCCCTCATCT
ACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGCCCTTCTTCAGT
CCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCC
ACTGAATATTGGATCACAAGGATCCACTGACTATTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMASAARLTMMWEEVTCPICLDPFVEPV
SIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLK
EISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVP
LEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSR
IHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQE
LISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPG
LKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDS
YPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGF
WTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHG
SLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15735
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 56532 Daltons