cDNA Clone USP52

• Synonyms: PAN2
• Plasmid: pFastbacDual 6P1 USP52
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST 3C USP52
• Genbank: NM_001127460.3
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCCATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGCCCCAGCCATGCATTCTGCCCTGGACCCTGTCTTGGATGCCCACCTGAACC
CAAGTCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGAGGCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGA
ATTGCACAGCGTGGTGGCTGAAGTGGGTGTACCTGTTTCCGTCTCCCACTTTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACGGGGGCCATGCC
ACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAATGGCAGTGATGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTT
TTCTCACCAAGAACAACCTCAAGTATATGGCCCGTGGGGGCCTCATTATATTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGAC
TGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGATTGATCTTAACACTGTCCAGGAGACTCAGAAGTATGCAGTAGAGACGCCT
GGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTCTGCGGCCACACGTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATG
AGTTTGATGCCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAACCTGCTAGCTGCCTGTGGCTTCTCCAGCCGCCTCACTGGCCTGGCCTGCGA
CCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCACTTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACT
TCTCGTCTTGCTATCATCTCTCAGTCAGGGCAGTGCCAATTCTGTGAACCCACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAATCCTGTGGGGC
CTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTTGGGGATTCTGAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTC
CTTCAACCCCTACTCCCGTGAGACTGAGTTTGCTTTGCCGTGTCTCGTGGACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCTC
ATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGCCAACTCTGCTCCAGCTCCCAGGCGAGCACCACCCGTGGATGCAGAGATTC
TGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGCCCAATCCCCGCACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGA
ATTTGACAGCTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGGAACCACATCTCCACATGGTTTCTAAGAAATACCGCAAGGTGACCATCAAA
TATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGACCTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCA
TGATCCAGGTGCTCTATTTCCTGGAGCCTGTACGCTGTCTAATTCAAAACCACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTGGGCTTCCTGTT
TCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTCTTCGGGCATTCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTG
GCTGACTCAGATGAGGCCTCAGGCAAGGGCAATCTGGCCAGGCTCATTCAGAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAGC
TGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCAGCTTTTGCTCATCGGGGGACTCTGTTATTGGGCAGCTCTTCAGCTGTGAGATGGAGAACTG
CAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTCATCCACTCTGCTTTTCACACTCTCCTACCCTGATGGTAGCAAAAGTGATAAAACTGGGAAG
AACTATGACTTTGCTCAGGTGCTGAAGCGAAGCATCTGCCTGGACCAGAATACACAGGCCTGGTGTGACACCTGTGAAAAGTACCAGCCCACGATTCAGA
CCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGTGAACAGCTCAAAAGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTT
CAAGATGGCAGTAAAGAAACACGGTGGGGAAATCTCCAAGAACAAGGAATTTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGGGTGTGCTGGTG
TGTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCGCATGAAGATGACCAAAAACAAAGGGCTGGATGTTTGCAATTGGACTGATG
GGGATGAGATGCAGTGGGGCCCAGCCAGGGCAGAGGAGGAGCATGGTGTCTATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCAC
AGGGGGCAGCCTGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGCGCAAGGAGGGCGTTACTCACCAGCAGTGGTATCTGTTCAATGACTTTCTT
ATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGACATGAATTGGAAAGTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACA
ACCTGAACATCAAGAACCCTATTGAGGCAAGTGTCTTGCTGGCTGAAGCCTCGCTGGCACGGAAGCAGCGGAAAACACATACTACCTTTATTCCACTGAT
GCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAGTTTGTCACCCTTAATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAG
TCTACCATTAAACCAAGCCAGATGTCAGTAGCCAGGATTACCTGTGTTCGGGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGACTACATCTCTA
CCCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCTGGTGACCTCGATGCCAAAATTTCCTCCAAGCACCTAACAACTCTCAAGTC
TACCTACTTAAAGCTTCGTTTTCTCATTGACATTGGAGTCAAGTTTGTGGGTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAAG
GACCAAGTCCTTGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAATGATTTCCCTGCGATTCCTTGCTTGGTACTTTCTGGACCTGAAGATTCAAG
GGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCCTTCAGCTGTACCGAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCA
CAAGGTGCTCAAGGGTCTTTATGAGAAGGGCAGAAAGATGGACTGGAAGGTGCCTGAGCCTGAGGGCCAAACAAGTCCCAAGAATGCAGCTGTCTTCTCC
TCAGTGCTGGCGCTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMNFEGLDPGLAEYAPAMHSALDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIM
EGVYSELHSVVAEVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYMARGGLIIFDYLLDENEDM
HSLLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFFCGHTSGKVSLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRL
TGLACDRFLKVYDLRMMRAITPLQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDSEGCVHLWT
DSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAPRRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRL
KESDSEFDSFSQVTESPVGREEEPHLHMVSKKYRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLAC
ELGFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQELEIPQAYRGAGGSSFCSSGDSVIGQLFS
CEMENCSLCRCGSETVRASSTLLFTLSYPDGSKSDKTGKNYDFAQVLKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRM
QAEVAFKMAVKKHGGEISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEEHGVYVYDLMATVVH
ILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHT
TFIPLMLNEMPQIGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKH
LTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGT
EPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVLAL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbacDual 6P1 USP5294.25 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU15686
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: poly(A) specific ribonuclease subunit PAN2. Compared to reference sequence there is a SNP at I179L (a535c).
• Low Temp: No