cDNA Clone USP7

• Plasmid: pFBHTb USP7
• Parent Plasmid: pFastBac HTb
• Expressed: 6His USP7
• Genbank: NP_003461.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACCACCAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCAGCAGTTGAGCGAGCCCGAGGACATGGAGATGGAAGCGGGAGATACAGATGACCCACCAAGAATTACTCAGAACCCTGTGATCAATGGGAATGTGGCCCTGAGTGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAGTTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGACTGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGGAAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAGCGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGTCTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAAAAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGATTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGAAAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCGGATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGACGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACCGAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTTCTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAAAGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAGGAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCACCTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCTATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTATTATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATTTGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACGCTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACATTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCAGACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGTTACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAATTATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACATTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAATTATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTACATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGGTCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAGAAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATATGCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGAATGACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTGAAGAACTCCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCATGGGATTTCCACAAGATCAAATTCGATTGTGGCCCATGCAAGCAAGGAGTAATGGAACAAAACGACCAGCAATGTTAGATAATGAAGCCGACGGCAATAAAACAATGATTGAGCTCAGTGATAATGAAAACCCTTGGACAATATTCCTGGAAACAGTTGATCCCGAGCTGGCTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAGATCATGATGTAATGTTATTTTTGAAGATGTATGATCCCAAAACGCGGAGCTTGAATTACTGTGGGCATATCTACACACCAATATCCTGTAAAATACGTGACTTGCTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGATACTAGCCTTATCCTCTATGAGGAAGTTAAACCGAATTTAACAGAGAGAATTCAGGACTATGACGTGTCTCTTGATAAAGCCCTTGATGAACTAATGGATGGTGACATCATAGTATTTCAGAAGGATGACCCTGAAAATGATAACAGTGAATTACCCACCGCAAAGGAGTATTTCCGAGATCTCTACCACCGCGTTGATGTCATTTTCTGTGATAAAACAATCCCTAATGATCCTGGATTTGTGGTTACGTTATCAAATAGAATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACACAGATCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGATGGCCCAGGTAATCCTCTTAGACATAATTATGAAGGTACTTTAAGAGATCTTCTACAGTTCTTCAAGCCTAGACAACCTAAGAAACTTTACTATCAGCAGCTTAAGATGAAAATCACAGACTTTGAGAACAGGCGAAGTTTTAAATGTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATATCCAGACAAGCATGGGTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAGGCCGTGGAGCTTGGGGAGAAAGCATCAGGGAAACTTAGGCTGCTAGAAATTGTAAGCTACAAAATCATTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTATCTCCTGCAACGAGCCGGACGTTTCGAATAGAGGAAATCCCTTTGGACCAGGTGGACATAGACAAAGAGAATGAGATGCTTGTCACAGTGGCGCATTTCCACAAAGAGGTCTTCGGAACGTTCGGAATCCCGTTTTTGCTGAGGATACACCAGGGCGAGCATTTTCGAGAAGTGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGGAGAAGGAGTTTGAGAAGTTTAAATTTGCAATTGTAATGATGGGCCGACACCAGTACATAAATGAAGACGAGTATGAAGTAAATTTGAAAGACTTTGAGCCACAGCCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTCGACCACTTCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTTGAAAAGGCCATTAAAATCCATAACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNHQQQQQQQKAGEQQLSEPED
MEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQ
FTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCN
AESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMA
WSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGAT
CYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDK
PVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPK
LFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQ
LDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKIN
DRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPK
GDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRES
KLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAE
DQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRL
WPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAAS
GATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCD
RAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDD
PENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQV
AKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQ
PKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLL
EECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRI
EEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMK
RIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSH
PRPWLGLDHFNKAPKRSRYTYLEKAIKIHN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15642
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 131671 Daltons