cDNA Clone DDX3X

• Plasmid: pMalP DDX3X
• Parent Plasmid: pMAL6P
• Expressed: MBP DDX3X
• Genbank: NM_001356
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCA
GTTTGCTGGCCTAGACCTGAACTCTTCAGATAATCAGAGTGGAGGAAGTA
CAGCCAGCAAAGGGCGCTATATTCCTCCTCATTTAAGGAACCGAGAAGCT
ACTAAAGGTTTCTACGATAAAGACAGTTCAGGGTGGAGTTCTAGCAAAGA
TAAGGATGCGTATAGCAGTTTTGGATCTCGTAGTGATTCAAGAGGGAAGT
CTAGCTTCTTCAGTGATCGTGGAAGTGGATCAAGGGGAAGGTTTGATGAT
CGTGGACGGAGTGATTACGATGGCATTGGCAGCCGTGGTGACAGAAGTGG
CTTTGGCAAATTTGAACGTGGTGGAAACAGTCGCTGGTGTGACAAATCAG
ATGAAGATGATTGGTCAAAACCACTCCCACCAAGTGAACGCTTGGAACAG
GAACTCTTTTCTGGAGGCAACACTGGGATTAATTTTGAGAAATACGATGA
CATTCCAGTTGAGGCAACAGGCAACAACTGTCCTCCACATATTGAAAGTT
TCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTGAGCTTACT
CGTTATACTCGCCCAACTCCAGTGCAAAAGCATGCTATTCCTATTATCAA
AGAGAAAAGAGACTTGATGGCTTGTGCCCAAACAGGGTCTGGAAAAACTG
CAGCATTTCTGTTGCCCATCTTGAGTCAGATTTATTCAGATGGTCCAGGC
GAGGCTTTGAGGGCCATGAAGGAAAATGGAAGGTATGGGCGCCGCAAACA
ATACCCAATCTCCTTGGTATTAGCACCAACGAGAGAGTTGGCAGTACAGA
TCTACGAGGAAGCCAGAAAATTTTCATACCGATCTAGAGTTCGTCCTTGC
GTGGTTTATGGTGGTGCCGATATTGGTCAGCAGATTCGAGACTTGGAACG
TGGATGCCATTTGTTAGTAGCCACTCCAGGACGTCTAGTGGATATGATGG
AAAGAGGAAAGATTGGATTAGACTTTTGCAAATACTTGGTGTTAGATGAA
GCTGATCGGATGTTGGATATGGGGTTTGAGCCTCAGATTCGTAGAATAGT
CGAACAAGATACTATGCCTCCAAAGGGTGTCCGCCACACTATGATGTTTA
GTGCTACTTTTCCTAAGGAAATACAGATGCTGGCTCGTGATTTCTTAGAT
GAATATATCTTCTTGGCTGTAGGAAGAGTTGGCTCTACCTCTGAAAACAT
CACACAGAAAGTAGTTTGGGTGGAAGAATCAGACAAACGGTCATTTCTGC
TTGACCTCCTAAATGCAACAGGCAAGGATTCACTGACCTTAGTGTTTGTG
GAGACCAAAAAGGGTGCAGATTCTCTGGAGGATTTCTTATACCATGAAGG
ATACGCATGTACCAGCATCCATGGAGACCGTTCTCAGAGGGATAGAGAAG
AGGCCCTTCACCAGTTCCGCTCAGGAAAAAGCCCAATTTTAGTGGCTACA
GCAGTAGCAGCAAGAGGACTGGACATTTCAAATGTGAAACATGTTATCAA
TTTTGACTTGCCAAGTGATATTGAAGAATATGTACATCGTATTGGTCGTA
CGGGACGTGTAGGAAACCTTGGCCTGGCAACCTCATTCTTTAACGAGAGG
AACATAAATATTACTAAGGATTTGTTGGATCTTCTTGTTGAAGCTAAACA
AGAAGTGCCGTCTTGGTTAGAAAACATGGCTTATGAACACCACTACAAGG
GTAGCAGTCGTGGACGTTCTAAGAGTAGCAGATTTAGTGGAGGGTTTGGT
GCCAGAGACTACCGACAAAGTAGCGGTGCCAGCAGTTCCAGCTTCAGCAG
CAGCCGCGCAAGCAGCAGCCGCAGTGGCGGAGGTGGCCACGGTAGCAGCA
GAGGATTTGGTGGAGGTGGCTATGGAGGCTTTTACAACAGTGATGGATAT
GGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGGGGTAACTGAGCGGC
CGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMSHVAVENALGLDQQFAGLDLN
SSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSF
GSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERG
GNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATG
NNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMA
CAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVL
APTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVA
TPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPP
KGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWV
EESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIH
GDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDI
EEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLE
NMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSR
SGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression.; ; Estimated MW of expressed protein : 119979 Daltons