cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pCMV-FLAG TPL2 S62A S400A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TPL2 S62A S400A
• Genbank: P41279
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTT
ATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAATC
TTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATG
TGTCAAGACAGTAATCAAAACGATGAGCGTTCTAAGGCTCTGCTGCTTAG
TGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATATGGAACTGTGGAGG
ATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTAT
GGACAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCC
CCAAAATGGACGTTACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGA
AGCTGACTTACAGGAATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTT
GGAAAGGTATACTTGGCTCAAGATATAAAGACGAAGAAAAGAATGGCGTG
TAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAAATTCAGG
CTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGG
GGTGAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCT
GGAGAAACTGGAGAGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGG
TGACAAAGCATGTTCTCAAGGGACTTGATTTTCTACACTCAAAGAAAGTG
ATCCATCATGATATTAAACCTAGCAACATTGTTTTCATGTCCACAAAAGC
TGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGATGTCTATT
TTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATC
CTGTGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCAC
GCTCATCCACATGCAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTC
GCTCAGCCTATCCCTCCTACCTGTACATAATCCACAAGCAAGCACCTCCA
CTGGAAGACATTGCAGATGACTGCAGTCCAGGGATGAGAGAGCTGATAGA
AGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCAGACCTAC
TAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTACG
GCTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGA
GCTGGAACTTCCTGAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCG
AGGAATCTGAGATGCTCAAGAGGCAACGCTCTCTCTACATCGACCTCGGC
GCTCTGGCTGGCTACTTCAATCTTGTTCGGGGACCACCAACGCTTGAATA
TGGCTGAGAATTC

Amino Acid Sequence

MDYKDDDDKGEYMSTGSDNKEEIDLLIKHLNVSDVIDIMENLYASEEPAV
YEPSLMTMCQDSNQNDERSKALLLSGQEVPWLSSVRYGTVEDLLAFANHI
SNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGS
DFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIA
ELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGL
DFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTE
IYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLY
IIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPP
REDQPRCTALDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKRQ
RSLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15435
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian N ter FLAG tagged construct.