cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pEBG6P-MEKK3
• Parent Plasmid: pEBG6P
• Expressed: GST MEKK3
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCT
GGTGGCCCTCCGGATGAACCGACGTCACCGGATGCCTGGATATGAGACCA
TGAAGAACAAAGACACAGGTCACTCAAATAGGCAGAGTGACGTCAGAATC
AAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCCTGT
GAAATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTC
TTGATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAA
GATGATCTTGATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAA
AAGCCTTAGGATATTGCTGTTGTCCCAGGACAGAAACCATAACAGTTCCT
CTCCCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCC
GCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAGGCA
CCTCTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCT
ATGTTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGC
ATCAACAGTGAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCT
GGATCCCCTGAGCAGTGCAGAAAATTCCTTGTCTGGAAGCTGCCAATCCT
TGGACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTC
CCTGACAACAGACAGGAATACTCAGATCGGGAAACTCAGCTTTATGACAA
AGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACC
ACAAGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGTCAT
CAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAA
TGGCGAGAACATGGGCCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCC
TGCGGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTG
CCAACCAAGTCTCCCAGTGCCCCCATCAACTGGCGCCGGGGAAAGCTCCT
GGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGG
GACGTGAACTTGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCTGAG
ACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAA
CTTGCAGCATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCG
CTGAGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTG
AAAGACCAGTTGAAGGCTTACGGTGCTCTGACAGAGAGCGTGACCCGAAA
GTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGA
TTGTTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCTGGG
AATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTG
TATGTCGGGGACGGGCATGCGCTCCGTCACTGGCACACCCTACTGGATGA
GCCCTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGG
AGCCTGGGCTGCACTGTGGTGGAGATGCTGACAGAGAAACCACCGTGGGC
AGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCA
ATCCTCAGCTGCCCTCCCACATCTCTGAACATGGCCGGGACTTCCTGAGG
CGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCAC
ACACCACTTTGCACAGCTCATGTACTGAGAATTCGCAAGGGCGAATTCGC
GGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDEQ
EALNSIMNDLVALRMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGE
RRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAI
DILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTI
YQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEF
IPETSEQCMLDPLSSAENSLSGSCQSLDSPSFRKSRMSRAQSFPDNRQEY
SDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTL
VPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSA
PINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSA
LECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAY
GALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDF
GASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVV
EMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEAR
QRPSAEELLTHHFAQLMY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15340
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 98699 Daltons