cDNA Clone ASK1

• Synonyms: MAP3K5
• Plasmid: pCMV5 HA2 ASK1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA ASK1
• Genbank: NP_005914
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGCACGGAGGCGGACGAGGGCATCACTTTCTCTGTGCC
ACCCTTCGCCCCCTCGGGCTTCTGCACCATCCCCGAGGGCGGCATCTGCA
GGAGGGGAGGAGCGGCGGCGGTGGGCGAGGGCGAGGAGCACCAGCTGCCA
CCGCCGCCGCCGGGCAGCTTCTGGAACGTGGAGAGCGCCGCTGCCCCTGG
CATCGGTTGTCCGGCGGCCACCTCCTCGAGCAGTGCCACCCGAGGCCGGG
GCAGCTCTGTTGGCGGGGGCAGCCGACGGACCACGGTGGCATATGTGATC
AACGAAGCGAGCCAAGGGCAACTGGTGGTGGCCGAGAGCGAGGCCCTGCA
GAGCTTGCGGGAGGCGTGCGAGACAGTGGGCGCCACCCTGGAAACCCTGC
ATTTTGGGAAACTCGACTTTGGAGAAACCACCGTGCTGGACCGCTTTTAC
AATGCAGATATTGCGGTGGTGGAGATGAGCGATGCCTTCCGGCAGCCGTC
CTTGTTTTACCACCTTGGGGTGAGAGAAAGTTTCAGCATGGCCAACAACA
TCATCCTCTACTGTGATACTAACTCGGACTCTCTGCAGTCACTGAAGGAA
ATAATTTGCCAGAAGAATACTATGTGCACTGGGAACTACACCTTTGTTCC
TTACATGATAACTCCACATAACAAAGTCTACTGCTGTGACAGCAGCTTCA
TGAAGGGGTTGACAGAGCTCATGCAACCGAACTTCGAGCTGCTTCTTGGA
CCCATCTGCTTACCTCTTGTGGATCGTTTTATTCAACTTTTGAAGGTGGC
ACAAGCAAGTTCTAGCCAGTACTTCCGGGAATCTATACTCAATGACATCA
GGAAAGCTCGTAATTTATACACTGGTAAAGAATTGGCAGCTGAGTTGGCA
AGAATTCGGCAGCGAGTAGATAATATCGAAGTCTTGACAGCAGATATTGT
CATAAATCTGTTACTTTCCTACAGAGATATCCAGGACTATGATTCTATTG
TGAAGCTGGTAGAGACTTTAGAAAAACTGCCAACCTTTGATTTGGCCTCC
CATCACCATGTGAAGTTTCATTATGCATTTGCACTGAATAGGAGAAATCT
CCCTGGTGACAGAGCAAAAGCTCTTGATATTATGATTCCCATGGTGCAAA
GCGAAGGACAAGTTGCTTCAGATATGTATTGCCTAGTTGGTCGAATCTAC
AAAGATATGTTTTTGGACTCTAATTTCACGGACACTGAAAGCAGAGACCA
TGGAGCTTCTTGGTTCAAAAAGGCATTTGAATCTGAGCCAACACTACAGT
CAGGAATTAATTATGCGGTCCTCCTCCTGGCAGCTGGACACCAGTTTGAA
TCTTCCTTTGAGCTCCGGAAAGTTGGGGTGAAGCTAAGTAGTCTTCTTGG
TAAAAAGGGAAACTTGGAAAAACTCCAGAGCTACTGGGAAGTTGGATTTT
TTCTGGGGGCCAGCGTCCTAGCCAATGACCACATGAGAGTCATTCAAGCA
TCTGAAAAGCTTTTTAAACTGAAGACACCAGCATGGTACCTCAAGTCTAT
TGTAGAGACAATTTTAATATATAAGCATTTTGTGAAACTGACCACAGAAC
AGCCTGTGGCCAAGCAAGAACTTGTGGACTTTTGGATGGATTTCCTGGTC
GAGGCCACAAAGACAGATGTTACTGTGGTTAGGTTTCCAGTATTAATATT
AGAACCAACCAAAATCTATCAACCTTCTTATTTGTCTATCAACAATGAAG
TTGAGGAAAAGACAATCTCTATTTGGCACGTGCTTCCTGATGACAAGAAA
GGTATACATGAGTGGAATTTTAGTGCCTCTTCTGTCAGGGGAGTGAGTAT
TTCTAAATTTGAAGAAAGATGCTGCTTTCTTTATGTGCTTCACAATTCTG
ATGATTTCCAAATCTATTTCTGTACAGAACTTCATTGTAAAAAGTTTTTT
GAGATGGTGAACACCATTACCGAAGAGAAGGGGAGAAGCACAGAGGAAGG
AGACTGTGAAAGTGACTTGCTGGAGTATGACTATGAATATGATGAAAATG
GTGACAGAGTCGTTTTAGGAAAAGGCACTTATGGGATAGTCTACGCAGGT
CGGGACTTGAGCAACCAAGTCAGAATTGCTATTAAGGAAATCCCAGAGAG
AGACAGCAGATACTCTCAGCCCCTGCATGAAGAAATAGCATTGCATAAAC
ACCTGAAGCACAAAAATATTGTCCAGTATCTGGGCTCTTTCAGTGAGAAT
GGTTTCATTAAAATCTTCATGGAGCAGGTCCCTGGAGGAAGTCTTTCTGC
TCTCCTTCGTTCCAAATGGGGTCCATTAAAAGACAATGAGCAAACAATTG
GCTTTTATACAAAGCAAATACTGGAAGGATTAAAATATCTCCATGACAAT
CAGATAGTTCACCGGGACATAAAGGGTGACAATGTGTTGATTAATACCTA
CAGTGGTGTTCTCAAGATCTCTGACTTCGGAACATCAAAGAGGCTTGCTG
GCATAAACCCCTGTACTGAAACTTTTACTGGTACCCTCCAGTATATGGCA
CCAGAAATAATAGATAAAGGACCAAGAGGCTACGGAAAAGCAGCAGACAT
CTGGTCTCTGGGCTGTACAATCATTGAAATGGCCACAGGAAAACCCCCAT
TTTATGAACTGGGAGAACCACAAGCAGCTATGTTCAAGGTGGGAATGTTT
AAAGTCCACCCTGAGATCCCAGAGTCCATGTCTGCAGAGGCCAAGGCATT
CATACTGAAATGTTTTGAACCAGATCCTGACAAGAGAGCCTGTGCTAACG
ACTTGCTTGTTGATGAGTTTTTAAAAGTTTCAAGCAAAAAGAAAAAGACA
CAACCTAAGCTTTCAGCTCTTTCAGCTGGATCAAATGAATATCTCAGGAG
TATATCCTTGCCGGTACCTGTGCTGGTGGAGGACACCAGCAGCAGCAGTG
AGTACGGCTCAGTTTCACCCGACACGGAGTTGAAAGTGGACCCCTTCTCT
TTCAAAACAAGAGCCAAGTCCTGCGGAGAAAGAGATGTCAAGGGAATTCG
GACACTCTTTTTGGGCATTCCAGATGAGAATTTTGAAGATCACAGTGCTC
CTCCTTCCCCTGAAGAAAAAGATTCTGGATTCTTCATGCTGAGGAAGGAC
AGTGAGAGGCGAGCTACCCTTCACAGGATCCTGACGGAAGACCAAGACAA
AATTGTGAGAAACCTAATGGAATCTTTAGCTCAGGGGGCTGAAGAACCGA
AACTAAAATGGGAACACATCACAACCCTCATTGCAAGCCTCAGAGAATTT
GTGAGATCCACTGACCGAAAAATCATAGCCACCACACTGTCAAAGCTGAA
ACTGGAGCTGGACTTCGACAGCCATGGCATTAGCCAAGTCCAGGTGGTAC
TCTTTGGTTTTCAAGATGCTGTCAATAAAGTTCTTCGGAATCATAACATC
AAGCCGCACTGGATGTTTGCCTTAGACAGTATCATTCGGAAGGCGGTACA
GACAGCCATTACCATCCTGGTTCCAGAACTAAGGCCACATTTCAGCCTTG
CATCTGAGAGTGATACTGCTGATCAAGAAGACTTGGATGTAGAAGATGAC
CATGAGGAACAGCCTTCAAATCAAACTGTCCGAAGACCTCAGGCTGTCAT
TGAAGATGCTGTGGCTACCTCAGGCGTGAGCACGCTCAGTTCTACTGTGT
CTCATGATTCCCAGAGTGCTCACCGGTCACTGAATGTACAGCTTGGAAGG
ATGAAAATAGAAACCAATAGATTACTGGAAGAATTGGTTCGGAAAGAGAA
AGAATTACAAGCACTCCTTCATCGAGCTATTGAAGAAAAAGACCAAGAAA
TTAAACACCTGAAGCTTAAGTCCCAACCCATAGAAATTCCTGAATTGCCT
GTATTTCATCTAAATTCTTCTGGCACAAATACTGAAGATTCTGAACTTAC
CGACTGGCTGAGAGTGAATGGAGCTGATGAAGACACTATAAGCCGGTTTT
TGGCTGAAGATTATACACTATTGGATGTTCTCTACTATGTTACACGTGAT
GACTTAAAATGCTTGAGACTAAGGGGAGGGATGCTGTGCACACTGTGGAA
GGCTATCATTGACTTTCGAAACAAACAGACTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMSTEADEGITFSVPPFAPSGFCTIPEG
GICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSAT
RGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATL
ETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSM
ANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCD
SSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESIL
NDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDY
DSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIP
MVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEP
TLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWE
VGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKL
TTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSI
NNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVL
HNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEY
DENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIA
LHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNE
QTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSK
RLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATG
KPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRA
CANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTS
SSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFED
HSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGA
EEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQV
QVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPH
FSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLS
STVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEK
DQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTI
SRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 156896 Daltons