cDNA Clone PI3K alpha; p85 (mouse)

• Plasmid: pFBDual 6His PI3K alpha, p85 (mouse)
• Parent Plasmid: pFastbacDual
• Expressed: 6His PI3Kalpha, p85
• Genbank: AAH89038 and AAH51106
• Tag: 6His
• Species: Mouse

Sequence of Insert

DNA sequence of PI3Kalpha :
GGATCCATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTT
GATGCCCCCACGAATCCTAGTGGAATGTTTACTCCCCAATGGAATGATAG
TGACTTTAGAATGCCTCCGTGAGGCCACACTCGTCACCATCAAACATGAA
CTGTTCAGAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGACGA
AACTTCTTACATTTTCGTAAGTGTCACCCAAGAAGCAGAAAGGGAAGAAT
TTTTTGATGAAACAAGACGACTTTGTGACCTTCGGCTTTTTCAACCCTTT
TTAAAAGTTATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCG
AGAAATTGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTA
AAGATCCAGAAGTCCAAGACTTTCGAAGGAACATTCTGAATGTTTGCAAA
GAAGCTGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAATGTA
TGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCT
ACAACAAGTTAGATAAAGGACAAATCATAGTGGTGATTTGGGTAATAGTC
TCTCCAAACAACGACAAGCAGAAGTACACTCTGAAGATCAATCATGACTG
TGTGCCAGAGCAAGTCATTGCTGAAGCCATCAGGAAAAAGACTCGGAGCA
TGTTGTTGTCCTCTGAGCAGCTGAAACTCTGTGTCTTAGAATATCAGGGC
AAGTATATTCTGAAAGTGTGTGGCTGTGACGAATACTTCCTGGAAAAGTA
CCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGGAGGA
TGCCCAACTTGATGCTGATGGCCAAAGAAAGCCTATACTCTCAGCTGCCG
ATTGATAGCTTCACCATGCCGTCATACTCCAGGCGCATCTCCACAGCCAC
ACCCTACATGAATGGAGAGACATCTACGAAATCCCTCTGGGTCATAAATA
GTGCGCTCAGAATAAAAATTCTTTGTGCAACCTATGTAAATGTAAATATT
CGAGACATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGA
ACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCTA
GGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCCTCGT
GCTGCGCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGC
TAAGGAGGAGCACTGTCCGTTGGCCTGGGGAAACATAAACTTGTTTGATT
ATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGAATCTCTGGCCTGTA
CCGCATGGGTTAGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAA
TCCAAATAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCA
GTGTGGTGAAGTTTCCAGACATGTCTGTGATCGAAGAACATGCCAATTGG
TCCGTGTCCCGAGAAGCTGGATTCAGTTACTCCCATACAGGACTGAGTAA
CAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCC
GAGCACTTTGCACCCGGGACCCACTATCTGAAATCACTGAACAAGAGAAA
GACTTCCTATGGAGCCACAGACACTACTGCGTAACTATTCCTGAAATCCT
ACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGACGAAGTGGCCC
AGATGTACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCC
ATGGAACTCCTGGACTGTAACTATCCAGATCCTATGGTTCGGAGTTTTGC
TGTTCGGTGCTTAGAAAAATATTTAACAGATGACAAACTTTCTCAGTACC
TCATTCAACTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTG
CTTGTGAGATTTTTACTCAAGAAAGCATTGACAAATCAAAGGATTGGCCA
TTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAATAAGACTGTCAGTC
AGAGGTTTGGCCTGCTATTGGAGTCCTACTGCCGTGCCTGTGGGATGTAT
CTGAAGCACCTGAACAGACAAGTAGAGGCCATGGAGAAGCTCATCAACCT
AACGGACATCCTTAAGCAGGAGAAGAAGGATGAGACACAAAAGGTACAGA
TGAAGTTTTTGGTTGAACAGATGAGACAGCCAGACTTCATGGATGCTTTG
CAGGGTTTTCTGTCCCCTCTGAATCCTGCTCACCAACTAGGAAACCTCAG
GCTTGAAGAGTGTCGAATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGA
ATTGGGAGAACCCAGACATCATGTCAGAGCTACTGTTTCAGAACAATGAG
ATCATCTTTAAAAATGGCGACGACTTACGGCAAGATATGTTAACCCTTCA
GATCATCCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTC
GCATGCTACCTTATGGCTGTCTATCCATTGGGGACTGTGTGGGTCTCATC
GAGGTGGTGAGAAACTCTCACACCATCATGCAAATCCAGTGCAAAGGAGG
CCTGAAGGGGGCGCTGCAGTTCAACAGCCACACACTGCATCAATGGCTCA
AGGACAAGAACAAGGGCGAGATATATGATGCAGCCATTGACCTGTTCACT
CGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATCTTGGGAATTGGAGA
CCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATA
TAGATTTTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAG
CGGGAACGTGTGCCATTTGTGTTGACACAGGATTTCTTGATTGTGATTAG
TAAGGGAGCACAAGAGTACACCAAGACCAGAGAGTTTGAGAGGTTTCAGG
AGATGTGTTACAAGGCTTACCTAGCAATTCGGCAGCATGCCAATCTCTTC
ATCAACCTTTTTTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAATC
TTTTGATGACATTGCATATATCCGAAAGACTCTAGCCTTGGACAAAACTG
AGCAAGAAGCTTTGGAATATTTCACAAAGCAAATGAATGATGCACATCAT
GGTGGATGGACGACAAAAATGGATTGGATCTTCCACACCATCAAGCAGCA
TGCTTTGAACTGAGCGGCCGC


DNA sequence of p85 insert
GGCTCGAGGAAACCATGTACACCACGGTTTGGACTATGGAAGACCTGGAC
TTAGAGTGTGCCAAGACAGATATAAACTGTGGCACAGACTTGGTGTTTTA
TATAGAAATGGATCCACCAGCACTGCCCCCCAAACCACCCAAGCCCACTA
CTGTAGCCAACAACAGCATGAACAACAATATGTCCTTGCAGGATGCTGAA
TGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAACTCCGAGA
CACTGCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAATGCACG
GCGATTACACTCTTACACTAAGGAAAGGAGGAAATAACAAATTAATCAAA
ATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTAACCTTCAA
CTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTTAGCTCAGT
ACAACCCCAAGCTGGATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAG
CAGGATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGGAAAAAATT
ACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGATAGAT
TATATGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAAAGAACG
GCTATCGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAATGCCA
AACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAAACGCGAAG
GCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATAAGCTGAAG
TCGCGTATCAGTGAGATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTT
GAAGAAGCAGGCAGCTGAGTACCGAGAGATCGACAAACGCATGAACAGTA
TTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAATACTTGATG
TGGCTGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTGGCTGGG
GAATGAAAATACCGAAGATCAATACTCCCTGGTAGAAGATGATGAGGATT
TGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCAACCGAAAC
AAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTCCTTGTCCG
GGAGAGCAGTAAGCAGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCG
AAGTCAAGCATTGCGTCATTAACAAGACTGCCACCGGCTATGGCTTTGCC
GAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTACATTATCA
ACACACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACACTAGCAT
ACCCAGTATATGCACAACAGAGGCGATGAGGTACC

Amino Acid Sequence

Expressed PI3K alpha Protein :
MSYYHHHHHHDYDIPTTENLYFQGAMGSMPPRPSSGELWGIHLMPPRILV
ECLLPNGMIVTLECLREATLVTIKHELFREARKYPLHQLLQDETSYIFVS
VTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIG
MPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVE
SSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIA
EAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKY
IRSCIMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGET
STKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVN
TQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPL
AWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPC
LELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNE
LRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSV
KWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKY
LTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLK
SEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQE
KKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIM
SSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMEN
IWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQF
NSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIM
VKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYT
KTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYI
RKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN*Ex

Expressed p85 protein.
MYTTVWTMEDLDLECAKTDINCGTDLVFYIEMDPPALPPKPPKPTTVANN
SMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTL
TLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKL
DVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEY
TRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKE
IQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDL
IQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHD
EKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHC
VINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYA
QQRR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15226
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression to express N ter 6His PI3Kalpha and untagged p85 from mouse.; ; Estimated MW of PI3Kalpha : 127781 Daltons; Estimated MW of p85 : 53397 Daltons