cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pCMV-FLAG TPL2 K167R
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TPL2 K167R
• Genbank: P41279
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTT
ATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAATC
TTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATG
TGTCAAGACAGTAATCAAAACGATGAGCGTTCTAAGTCTCTGCTGCTTAG
TGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATATGGAACTGTGGAGG
ATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTAT
GGACAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCC
CCAAAATGGACGTTACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGA
AGCTGACTTACAGGAATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTT
GGAAAGGTATACTTGGCACAAGATATAAAGACGAAGAAAAGAATGGCGTG
TAGACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAAATCCAGG
CTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGG
GGTGAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCT
GGAGAAACTGGAGAGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGG
TGACAAAGCATGTTCTCAAGGGACTTGATTTTCTACACTCAAAGAAAGTG
ATCCATCATGATATTAAACCTAGCAACATTGTTTTCATGTCCACAAAAGC
TGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGATGTCTATT
TTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATC
CTGTGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCAC
GCTCATCCACATGCAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTC
GCTCAGCCTATCCCTCCTACCTGTACATAATCCACAAGCAAGCACCTCCA
CTGGAAGACATTGCAGATGACTGCAGTCCAGGGATGAGAGAGCTGATAGA
AGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCAGACCTAC
TAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTACG
AGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGA
GCTGGAACTTCCTGAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCG
AGGAATCTGAGATGCTCAAGAGGCAACGCTCTCTCTACATCGACCTCGGC
GCTCTGGCCGGCTACTTCAATCTTGTTCGGGGACCACCAACGCTTGAATA
TGGCTGAGAATTCCCGGGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSEYMSTGSDNKEEIDLLIKHLNVSDVIDIMENLYASEEPA
VYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANH
ISNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIG
SDFIPRGAFGKVYLAQDIKTKKRMACRLIPVDQFKPSDVEIQACFRHENI
AELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKG
LDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGT
EIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYL
YIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNP
PREDQPRCTSLDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKR
QRSLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 54063 Daltons