cDNA Clone TBK1; SINTABD

• Plasmid: pFB Dual GST TBK1, SINTBAD
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST TBK1, untagged SINTBAD
• Genbank: NP_037386
• Tag: untagged
• Species: Human

Sequence of Insert

DNA sequence of TBK1 insert
GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGC

DNA Sequence of SINTBAD
GGCTCGAGGAAACCATGGAGTCCATGTTTGAAGATGACATCAGCATCCTG
ACCCAAGAGGCCTTGGGGCCCAGTGAGGTGTGGCTGGATGGTCCTGGAGA
CCCGTCCCTGGGAGGGGACATGTGTTCTGCCTCCCACTTTGCCCTCATCA
CAGCCTACGGAGACATCAAGGAAAGGCTAGGAGGCCTGGAGAGGGAGAAT
GCCACCCTCCGCCGTCGCCTCAAAGTCTATGAGATCAAGTATCCGCTGAT
CACTGATTTTGGAGAGGAGCATGGCTTCCCTCTGTATGAACTCAAGGATG
GCTCCCTGCTGGAGGTGGAGAAGGTCAGTCTGCAGCAACGACTCAACCAA
TTCCAACACGAGTTGCAGAAGAGTAAGGAGCAGGAGGAGCAGCTCGGGGA
GATGATCCAGGCTTACGAGAAACTGTGCGTGGAGAAAAGCGACTTGGAGA
CAGAGCTGGGGGAGATGCGGGCCCTGGTAGAGACTCATCTACGGCAGATC
TGTGGTCTGGAGAAACAGCTGCAGCAACAGCAGGGCCTCCGGGACGCAGC
TTTCTCCAGCCTGAGCCCCCCAGCTGTCCCCGCCTCAGCCTGTCCTGACT
TGGACCTGCACTATCTGGCTCTGAGAGGGGGACCTGCCTTGGGTCATGGT
TGGCCTGGCCCTACGAGTGTAAGTGTGAGCGAGCTGGAGAGGAGGCGCCT
GGAAGAGGCCCTGGAGGCTGCGCAGGGGGAGGCTCGGGGGGCTCAGCTCC
GGGAGGAGCAACTCCAGGCGGAATGCGAGAGACTTCAGGGAGAGCTGAAG
CAACTGCAGGAGACCCGGGCCCAGGATCTGGCCTCCAACCAGTCGGAATG
TGACATGGCTTGGGTCAAGAGAGTTGGGGATGATCAGGTGAACTTGGCGC
TGGCCTACACAGAACTGACAGAGGAGCTGGGTCGGCTTCGGGAGCTGAGT
TCCCTGCAGGGGAGGATCTTGAGGACTTTGCTGCAGGAACAGGCCCGCAA
CGCAGGCCAAAGGCACTCACCGCTGTCGCAACGTCACTCCCCGGCCCCTG
CGTGCCCCTCACCCTCCCCGCCTGCCCGACCGCCGCCCTGTGCCCCGTGC
CCTTCGCCCCAGCAGCGCCGCTCGCCCGCCTCGCCGTCCTGCCCGTCCCC
AGTCCCACAGCGCCGCTCGCCCGTGCCGCCGTCGTGCCAGTCGCCCAGTC
CTCAGCGCCGCTCGCCTGTGCCACCCAGCTGCCCGGCCCCGCAGCCCCGG
CCGCCGCCACCACCGGGGGAGAGGACGCTGGCCGAGCGTGTCTACGCCAA
GCCGCCCAGCCACCACGCCAAGGCCGGCTTCCAGGGCCGCCGCAGCTACT
CGGAGCTGGCGGAGGGGGCGGCCTACGCTGGTGCCTCCCCCGCCTGGCTA
CAGGCCGAGGCTGCCACACTTCCCAAGCCCCGCGCCTATGGCGGCGAGCT
CTACGGCAGGCCGCTCAGCCCGCGCCGAGCCTTTGAGGGCATCCGCCTGC
GCTTTGAGAAGCAGCCATCTGAAGAAGAGGAGTGGGCTATGCCGGCCAGC
CCGCCCAGTCCCGAAGCGGGCACCATCCGCTGCGCTTCATTCTGTGCGGG
CTTCCCCATCCCAGAGTCGCCTGCGGCCACCGCCTATGCCCACGCTGAGC
ACGCACAGTCCTGGCCATCCATCAACCTACTGATGGAGACGGTGGGCTCT
GATATCCGCAGCTGCCCCCTCTGCCAGCTGGGTTTCCCTGTTGGGTACCC
AGATGATGCTCTCATCAAACACATAGACTCCCACCTTGAGAACAGCAAGA
TCTAGGCTAGCCC

Amino Acid Sequence

Protein Sequence of TBK1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTSNHLWLLSDILGQGA
TANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVK
LFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDV
VGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQ
FVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSL
PFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSL
SRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQM
TAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQH
FPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAIT
GVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLD
FCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSL
QDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKD
KAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEW
IRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGI
KHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTM
DGGLRNVDCL*

Protein sequence of SINTBAD
MESMFEDDISILTQEALGPSEVWLDGPGDPSLGGDMCSASHFALITAYGD
IKERLGGLERENATLRRRLKVYEIKYPLITDFGEEHGFPLYELKDGSLLE
VEKVSLQQRLNQFQHELQKSKEQEEQLGEMIQAYEKLCVEKSDLETELGE
MRALVETHLRQICGLEKQLQQQQGLRDAAFSSLSPPAVPASACPDLDLHY
LALRGGPALGHGWPGPTSVSVSELERRRLEEALEAAQGEARGAQLREEQL
QAECERLQGELKQLQETRAQDLASNQSECDMAWVKRVGDDQVNLALAYTE
LTEELGRLRELSSLQGRILRTLLQEQARNAGQRHSPLSQRHSPAPACPSP
SPPARPPPCAPCPSPQQRRSPASPSCPSPVPQRRSPVPPSCQSPSPQRRS
PVPPSCPAPQPRPPPPPGERTLAERVYAKPPSHHAKAGFQGRRSYSELAE
GAAYAGASPAWLQAEAATLPKPRAYGGELYGRPLSPRRAFEGIRLRFEKQ
PSEEEEWAMPASPPSPEAGTIRCASFCAGFPIPESPAATAYAHAEHAQSW
PSINLLMETVGSDIRSCPLCQLGFPVGYPDDALIKHIDSHLENSKI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15114
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminal GST TBK1, untagged SINTBAD; for baculovirus expression.; ; EStimated MW of TBK1 : 110463 Daltons; Estimated MW of SINTBAD : 65451 Daltons