cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pCMV5 GSK3b K85R
• Parent Plasmid: pCMV5
• Expressed: EE GSK3b K85R
• Genbank: L33801
• Tag: EE
• Species: Human

Sequence of Insert

GAATTCAGATCTGCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCC
CAGAACCACCTCCTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAG
CTTTTGGCAGCATGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACA
ACAGTGGTGGCAACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAG
CTATACAGACACTAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATC
AAGCCAAACTTTGTGATTCAGGAGAACTGGTCGCCATCAGGAAAGTATTG
CAGGACAAGAGATTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGA
TCACTGTAACATAGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGA
AGAAAGATGAGGTCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACA
GTATACAGAGTTGCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGT
GATTTATGTCAAGTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATA
TCCATTCCTTTGGAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTG
TTGGATCCTGATACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAA
GCAGCTGGTCCGAGGAGAACCCAATGTTTCGTATATCTGTTCTCGGTACT
ATAGGGCACCAGAGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATA
GATGTATGGTCTGCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACC
AATATTTCCAGGGGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGG
TCCTGGGAACTCCAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTAC
ACAGAATTTAAATTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTT
CCGACCCCGAACTCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGG
AGTATACACCAACTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCA
TTTTTTGATGAATTACGGGACCCAAATGTCAAACATCCAAATGGGCGAGA
CACACCTGCACTCTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCAC
CTCTGGCTACCATCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCT
TCAACCCCCACAAATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCG
TGGACAGACCAATAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGAA
CAGTCCCGAGCAGCCAGCTGCACAGGAAAAACCACCAGTTACTTGAGTGT
CACTCAGCAACACTGGTCACGTTTGGAAAGAATATTAAAAAAAAAAAAAA
AAGGAATTC

Amino Acid Sequence

MEFMPMESGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATP
GQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIRKVLQDKRFK
NRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVAR
HYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTA
VLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAG
CVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFP
QIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELR
DPNVKHPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNA
TAASDANTGDRGQTNNAASASASNST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15022
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal EE tag for mammalian expression.; ; Estimated MW of expressed protein : 47559 Daltons