cDNA Clone SIK (mouse)

• Plasmid: pEBG6P-SIK (mouse)
• Parent Plasmid: pEBG6P
• Expressed: GST SIK
• Genbank: NM_010831
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGTGATCATGTCGGAGTTCAGTGCGGTCCCCTCAGGCACTGG
CCAGGGCCAGCAGAAGCCCCTCCGGGTGGGCTTTTACGACGTGGAACGGA
CCCTGGGCAAAGGCAATTTTGCAGTGGTTAAGCTGGCGCGGCACCGAGTC
ACCAAAACGCAGGTTGCAATAAAAATAATTGACAAGACACGGTTAGATTC
TAGCAATCTGGAGAAGATCTACCGGGAGGTCCAGCTCATGAAACTTTTGA
ACCACCCAAATATCATCAAGCTTTATCAGGTTATGGAGACAAAGGATATG
CTCTACATTGTCACAGAATTTGCAAAAAATGGAGAAATGTTTGATTATCT
GACTTCCAACGGGCACTTGAGTGAAAACGAGGCTAGGCAGAAGTTCTGGC
AGATTCTGTCAGCCGTGGAGTACTGCCACAACCACCACATTGTCCACCGG
GACCTCAAGACGGAGAACCTGCTCCTGGACAGCAACATGGATATCAAGCT
AGCAGATTTTGGATTTGGGAATTTCTACAAGCCAGGCGAGCCTCTGTCCA
CGTGGTGTGGGAGCCCCCCGTATGCAGCCCCTGAAGTCTTCGAGGGGAAG
GAGTACGAGGGTCCCCAGCTGGACGTCTGGAGCCTCGGTGTGGTGCTGTA
CGTCCTGGTCTGTGGCTCCCTCCCCTTCGACGGGCCCAACCTGCCTACGC
TGAGACAGCGGGTACTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCT
CAAGACTGTGAGACGCTGATCAGACGCATGCTGGTCGTGGACCCCGCTAA
GCGCATCACCATAGCCCAGATCCGCCAGCACCGGTGGATGCAGGCCGACC
CCACTCTCCTGCAGCAGGATGACCCTGCCTTCGACATGCAAGGCTACACC
TCCAACCTGGGCGACTACAACGAACAGGTGCTAGGGATCATGCAGGCCCT
CGGCATCGACCGGCAGCGGACTATAGAGTCTCTGCAGAACAGCAGCTACA
ACCACTTTGCCGCCATTTACTACCTCCTACTCGAGCGCCTCAAGGAGCAT
CGAAGCGCCCAGCCCTCATCCCGGCCCACCCCTGCACCCACCAGACAGCC
CCAGCTCCGAAGCTCAGACCTCAGCAGTCTGGAGGTTCCTCAAGAAATTC
TCCCGTGTGACCCTTTCCGGCCCTCTCTGCTGTGCCCACAGCCCCAGGCC
TTGGCTCAGTCTGTCCTGCAGGCTGAGATAGACTGTGATCTCCACAGCTC
ACTTCAGCCCTTATTATTCCCCCTGGATACCAACTGCAGTGGAGTGTTCC
GGCACCGATCCATCTCCCCCAGCAGTCTGCTGGACACAGCTATCAGCGAG
GAGGCCAGGCAGGGTCCCAGCCTAGAGGAGGAACAGGAGGTCCAGGAACC
CCTGCCCGGAAGCACAGGCCGGAGGCACACATTGGCTGAAGTCTCCACCC
ATTTCTCCCCGCTCAACCCTCCTTGCATAATTGTCTCCTCCTCTGCCACG
GCGAGTCCCTCGGAAGGGACCAGCTCCGACAGCTGCCTCCCCTTCTCTGC
AAGTGAAGGTCCTGCAGGGCTTGGCAGTGGCCTGGCCACCCCAGGGCTGC
TGGGCACCAGCTCTCCAGTCAGATTGGCCTCGCCCTTCCTGGGATCACAG
TCAGCCACCCCTGTGCTCCAGACTCAGGCAGGTCTGGGCACAGCCGTCTT
ACCTCCCGTCAGCTTCCAGGAGGGACGGAGAGCGTCTGATACCTCTCTCA
CTCAGGGGCTGAAGGCCTTCCGGCAGCAGCTGAGGAAAAATGCGAGGACC
AAGGGGTTCCTGGGACTGAACAAGATCAAAGGGTTGGCTCGCCAGGTGTG
CCAGTCCTCCGTCCGAACTCCCCGGGGAGGGATGAGTACCTTCCACACCC
CAGCCCCAAGCTCCGGCCTGCAAGGCTGCACGACCAGCAATCGGGAGGGC
AGGAGCCTACTTGAAGAGGTCCTGCACCAGCAGAGGCTGCTCCAGTTACA
GCACCACTCTTCTACCGCCGCCGCCTCATCTGGCTGCCAGCAGGGCCCCC
AGCTGTCCCCGGTCCCCTATGTGCTTGCTCCCTGTGACAGCCTCCTCGTG
TCTGGAATTCCACTGCTGCCAACACCCCTTCTCCAGGCTGGCATGTCCCC
TGTGGCGTCTGCTGCGCATCTCCTGGATACCCACTTGCACATCAGTGCTG
GCCCAGTGGCCCTCCCCACTGGGCCCCTGCCACAGTGCCTCACCAGGCTG
TCCCCAGGCTGTGATCCAGCTGGGCTGCCACAGGGGGACTGTGAGATGGA
GGACCTGACCTCTGGCCAGCGGGGGACATTCGTCCTGGTACAGTGAGCGG
CCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVIMSEFSAVPSG
TGQGQQKPLRVGFYDVERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRL
DSSNLEKIYREVQLMKLLNHPNIIKLYQVMETKDMLYIVTEFAKNGEMFD
YLTSNGHLSENEARQKFWQILSAVEYCHNHHIVHRDLKTENLLLDSNMDI
KLADFGFGNFYKPGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDVWSLGVV
LYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCETLIRRMLVVDP
AKRITIAQIRQHRWMQADPTLLQQDDPAFDMQGYTSNLGDYNEQVLGIMQ
ALGIDRQRTIESLQNSSYNHFAAIYYLLLERLKEHRSAQPSSRPTPAPTR
QPQLRSSDLSSLEVPQEILPCDPFRPSLLCPQPQALAQSVLQAEIDCDLH
SSLQPLLFPLDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQ
EPLPGSTGRRHTLAEVSTHFSPLNPPCIIVSSSATASPSEGTSSDSCLPF
SASEGPAGLGSGLATPGLLGTSSPVRLASPFLGSQSATPVLQTQAGLGTA
VLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQ
VCQSSVRTPRGGMSTFHTPAPSSGLQGCTTSNREGRSLLEEVLHQQRLLQ
LQHHSSTAAASSGCQQGPQLSPVPYVLAPCDSLLVSGIPLLPTPLLQAGM
SPVASAAHLLDTHLHISAGPVALPTGPLPQCLTRLSPGCDPAGLPQGDCE
MEDLTSGQRGTFVLVQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15007
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 112529 Daltons