cDNA Clone Pellino2

• Plasmid: pGEX6P-1-Pellino2 new
• Parent Plasmid: pGEX6P1
• Expressed: GST Pellino2
• Genbank: AAH09476.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGA
GCCAGTGAAATACGGGGAGCTGGTGGTGCTCGGGTACAATGGTGCTTTAC
CCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAAGCGG
CCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCC
CCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCCTACA
CTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGAT
ACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGT
TGTCACAGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCA
CACAGAGCACCATATCCAGGTTCGCCTGCAGGATCGTGTGCGACAGGAAT
GAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCAAAAA
CATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACA
TGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGC
TTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGG
AGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGC
TGGTGGAAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTG
TGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGGCTTTTTCATACTCC
AACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCCCGGC
CTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGG
AAAGAGGTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCA
CGTGCACGGGTACCACAACTGGGGCCATCGGAGTGACACGGAGGCCAACG
AGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGG
CTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGC
TTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATACTGGT
CTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCT
TTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTT
CCAAGGTCCAATTGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFSPGQEEHCAPNKEPVKY
GELVVLGYNGALPNGDRGRRKSRFALYKRPKANGVKPSTVHVISTPQASK
AISCKGQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPIDFVVTDT
ISGSQNTDEAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLG
EKAAKWKNPDGHMDGLTTNGVLVMHPRGGFTEESQPGVWREISVCGDVYT
LRETRSAQQRGKLVESETNVLQDGSLIDLCGATLLWRTADGLFHTPTQKH
IEALRQEINAARPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGY
HNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPC
GHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPI
D*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15004
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 73257 Daltons