cDNA Clone PPM1E

• Plasmid: pEBG6P-PPM1E
• Parent Plasmid: pEBG6P
• Expressed: GST PPM1E
• Genbank: AAI51229.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCT
GGAGCTATTCCTGGGCGAGTTTCGCGGACCGTGCGGCGGCGGCGAGCCGG
AGCCGGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAGTCCGAG
CCCGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAGGCTTCGGT
AGAGGAACCCGGGGAGGAGGCGGCCACGGTAGCCGCGACGGAGGAGGGGG
ACCAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTGAGGGTGAG
GAGGAGGAGGAGGGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGC
CGTGCCGCCGCCGCCGCCCCAGCTGCCGCCTTTGCCCCCGCTCCCGCGAC
CGCTGTCAGAGCGCATCACCCGCGAGGAGGTGGAGGGCGAAAGCCTGGAC
CTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTTTTGGCTGC
TGCTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTG
CACATTATATCCCAAAGGAAACGGATGGCACAGAAGGGACTGTGGAGATT
GAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACACGAGATTTG
CTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAGGAGACCCCAGCTTTATT
ATGAGACATCAATCCATGCCATCAAAAACATGCGCAGGAAAATGGAGGAC
AAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTAGAGGACCA
GGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATG
CTGCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAG
ATGTTCCCCCATGATCCTGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCAC
TGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAGATGTGGGA
CCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGTGGCCTGG
GTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACT
AATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATTGAGG
CCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGT
CTGTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCATATATCTG
TGGGGATGCAGATTCTGCCTCCACTGTTCTGGATGGGACCGAAGACTACC
TCATTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTGATGAGGCA
GTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACAGCAGCAT
GGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATA
ACATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGCTGTAAAT
GTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGA
AGATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCAAACGCCCTTGGC
CTCAGCACCAGTGCTCAGCACCAGCCGACCTAGGCTATGATGGGCGTGTG
GATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAAATCAACGT
GCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAAC
CTCACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCA
CCAAAGAAAGCAAATCTTATTAATGAGTTAATGATGGAGAAAAAATCAGT
TCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGTTTCCCACTGCTT
TCAATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAGCTTGTCT
CCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATCCCCGGGAAACAG
AGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAGTGGCACA
GATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCT
TCCCACAAAATAGGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAG
AAATAGGATAAGAAGTTCTCTGCCATGGAGGCAAAATAGTTGGAAAGGGT
ACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGATATTCCATGCCCA
GATCTTCCTTGGAGCTATAAAATAGAATAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAGCIPEEKTYRR
FLELFLGEFRGPCGGGEPEPEPEPEPEPEPEPESEPEPEPELVEAEAAEA
SVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGH
SAVPPPPPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFL
AAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLHE
ICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLE
DQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFR
VTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAV
ELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPY
ICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDS
SMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGG
QEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQI
NVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKK
SVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPG
NRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSG
KRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12995
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 111591 Daltons