cDNA Clone PI3K alpha (mouse)

• Plasmid: pFBHTb PI3K alpha (mouse)
• Parent Plasmid: pFastBac HTb
• Expressed: 6His PI3K alpha
• Genbank: AAH89038
• Tag: 6His
• Species: Mouse

Sequence of Insert

GGATCCATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTT
GATGCCCCCACGAATCCTAGTGGAATGTTTACTCCCCAATGGAATGATAG
TGACTTTAGAATGCCTCCGTGAGGCCACACTCGTCACCATCAAACATGAA
CTGTTCAGAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGACGA
AACTTCTTACATTTTCGTAAGTGTCACCCAAGAAGCAGAAAGGGAAGAAT
TTTTTGATGAAACAAGACGACTTTGTGACCTTCGGCTTTTTCAACCCTTT
TTAAAAGTTATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCG
AGAAATTGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTA
AAGATCCAGAAGTCCAAGACTTTCGAAGGAACATTCTGAATGTTTGCAAA
GAAGCTGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAATGTA
TGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCT
ACAACAAGTTAGATAAAGGACAAATCATAGTGGTGATTTGGGTAATAGTC
TCTCCAAACAACGACAAGCAGAAGTACACTCTGAAGATCAATCATGACTG
TGTGCCAGAGCAAGTCATTGCTGAAGCCATCAGGAAAAAGACTCGGAGCA
TGTTGTTGTCCTCTGAGCAGCTGAAACTCTGTGTCTTAGAATATCAGGGC
AAGTATATTCTGAAAGTGTGTGGCTGTGACGAATACTTCCTGGAAAAGTA
CCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGGAGGA
TGCCCAACTTGATGCTGATGGCCAAAGAAAGCCTATACTCTCAGCTGCCG
ATTGATAGCTTCACCATGCCGTCATACTCCAGGCGCATCTCCACAGCCAC
ACCCTACATGAATGGAGAGACATCTACGAAATCCCTCTGGGTCATAAATA
GTGCGCTCAGAATAAAAATTCTTTGTGCAACCTATGTAAATGTAAATATT
CGAGACATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGA
ACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCTA
GGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCCTCGT
GCTGCGCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGC
TAAGGAGGAGCACTGTCCGTTGGCCTGGGGAAACATAAACTTGTTTGATT
ATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGAATCTCTGGCCTGTA
CCGCATGGGTTAGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAA
TCCAAATAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCA
GTGTGGTGAAGTTTCCAGACATGTCTGTGATCGAAGAACATGCCAATTGG
TCCGTGTCCCGAGAAGCTGGATTCAGTTACTCCCATACAGGACTGAGTAA
CAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCC
GAGCACTTTGCACCCGGGACCCACTATCTGAAATCACTGAACAAGAGAAA
GACTTCCTATGGAGCCACAGACACTACTGCGTAACTATTCCTGAAATCCT
ACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGACGAAGTGGCCC
AGATGTACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCC
ATGGAACTCCTGGACTGTAACTATCCAGATCCTATGGTTCGGAGTTTTGC
TGTTCGGTGCTTAGAAAAATATTTAACAGATGACAAACTTTCTCAGTACC
TCATTCAACTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTG
CTTGTGAGATTTTTACTCAAGAAAGCATTGACAAATCAAAGGATTGGCCA
TTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAATAAGACTGTCAGTC
AGAGGTTTGGCCTGCTATTGGAGTCCTACTGCCGTGCCTGTGGGATGTAT
CTGAAGCACCTGAACAGACAAGTAGAGGCCATGGAGAAGCTCATCAACCT
AACGGACATCCTTAAGCAGGAGAAGAAGGATGAGACACAAAAGGTACAGA
TGAAGTTTTTGGTTGAACAGATGAGACAGCCAGACTTCATGGATGCTTTG
CAGGGTTTTCTGTCCCCTCTGAATCCTGCTCACCAACTAGGAAACCTCAG
GCTTGAAGAGTGTCGAATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGA
ATTGGGAGAACCCAGACATCATGTCAGAGCTACTGTTTCAGAACAATGAG
ATCATCTTTAAAAATGGCGACGACTTACGGCAAGATATGTTAACCCTTCA
GATCATCCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTC
GCATGCTACCTTATGGCTGTCTATCCATTGGGGACTGTGTGGGTCTCATC
GAGGTGGTGAGAAACTCTCACACCATCATGCAAATCCAGTGCAAAGGAGG
CCTGAAGGGGGCGCTGCAGTTCAACAGCCACACACTGCATCAATGGCTCA
AGGACAAGAACAAGGGCGAGATATATGATGCAGCCATTGACCTGTTCACT
CGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATCTTGGGAATTGGAGA
CCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATA
TAGATTTTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAG
CGGGAACGTGTGCCATTTGTGTTGACACAGGATTTCTTGATTGTGATTAG
TAAGGGAGCACAAGAGTACACCAAGACCAGAGAGTTTGAGAGGTTTCAGG
AGATGTGTTACAAGGCTTACCTAGCAATTCGGCAGCATGCCAATCTCTTC
ATCAACCTTTTTTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAATC
TTTTGATGACATTGCATATATCCGAAAGACTCTAGCCTTGGACAAAACTG
AGCAAGAAGCTTTGGAATATTTCACAAAGCAAATGAATGATGCACATCAT
GGTGGATGGACGACAAAAATGGATTGGATCTTCCACACCATCAAGCAGCA
TGCTTTGAACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMPPRPSSGELWGIHLMPPRILV
ECLLPNGMIVTLECLREATLVTIKHELFREARKYPLHQLLQDETSYIFVS
VTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIG
MPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVE
SSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIA
EAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKY
IRSCIMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGET
STKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVN
TQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPL
AWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPC
LELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNE
LRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSV
KWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKY
LTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLK
SEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQE
KKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIM
SSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMEN
IWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQF
NSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIM
VKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYT
KTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYI
RKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12977
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 127781 Daltons