cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pCMV-FLAG TPL2 30-397 S36A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TPL2 30-397 S36A
• Genbank: P41279
• Tag: FLAG

Sequence of Insert

GGATCCATGGAAAATCTTTATGCAGCTGAAGAGCCAGCAGTTTATGAACC
CAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCTA
AGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGA
TATGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACAC
TGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAA
ACATGGTCATCACTCCCCAAAATGGACGTTACCAAATAGATTCCGATGTT
CTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGTTCTGATTTTAT
TCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCTCAAGATATAAAGACGA
AGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCT
GATGTGGAAATTCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTA
TGGCGCAGTCCTGTGGGGTGAAACTGTCCATCTCTTTATGGAAGCAGGCG
AGGGAGGGTCTGTTCTGGAGAAACTGGAGAGCTGTGGACCAATGAGAGAA
TTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGACTTGATTTTCT
ACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTTT
TCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATG
ACCGAAGATGTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACAT
GAGCCCAGAGGTCATCCTGTGCAGGGGCCATTCAACCAAAGCAGACATCT
ACAGCCTGGGGGCCACGCTCATCCACATGCAGACGGGCACCCCACCCTGG
GTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTGTACATAATCCA
CAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGGA
TGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCA
AGAGCCGCAGACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGA
TCAGCCACGCTAAGAATTCCGCAAGGGCGAATTCTGCAGATATCCATCAC
ACTGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMENLYAAEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQ
EVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQN
GRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKL
IPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEK
LESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVL
VDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLI
HMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEAS
LERNPNHRPRAADLLKHEALNPPREDQPR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal FLAG tag.; ; Estimated MW of expressed protein : 43056 Daltons