cDNA Clone USP28

• Plasmid: pET156P USP28 1-582
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His USP28 1-582
• Genbank: AAH65928.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACTGCGGAGCTGCAGCAGGACGACGCGGCCGGCGCGGCAGA
CGGCCACGGCTCGAGCTGCCAAATGCTGTTAAATCAACTGAGAGAAATCA
CAGGCATTCAGGACCCTTCCTTTCTCCATGAAGCTCTGAAGGCCAGTAAT
GGTGACATTACTCAGGCAGTCAGCCTTCTCACTGATGAGAGAGTTAAGGA
GCCCAGTCAAGACACTGTTGCTACAGAACCATCTGAAGTAGAGGGGAGTG
CTGCCAACAAGGAAGTATTAGCAAAAGTTATAGACCTTACTCATGATAAC
AAAGATGATCTTCAGGCTGCCATTGCTTTGAGTCTACTGGAGTCTCCCAA
AATTCAAGCTGATGGAAGAGATCTTAACAGGATGCATGAAGCAACCTCTG
CAGAAACTAAACGCTCAAAGAGAAAACGCTGTGAAGTCTGGGGAGAAAAC
CCCAATCCCAATGACTGGAGGAGAGTTGATGGTTGGCCAGTTGGGCTGAA
AAATGTTGGCAATACATGTTGGTTTAGTGCTGTTATTCAGTCTCTCTTTC
AATTGCCTGAATTTCGAAGACTTGTTCTCAGTTATAGTCTGCCACAAAAT
GTACTTGAAAATTGTCGAAGTCATACAGAAAAGAGAAATATCATGTTTAT
GCAAGAGCTTCAGTATTTGTTTGCTCTAATGATGGGATCAAATAGAAAAT
TTGTAGACCCGTCTGCAGCCCTGGATCTATTAAAGGGAGCATTCCGATCA
TCTGAGGAACAGCAGCAAGATGTGAGTGAATTCACACACAAGCTCCTGGA
TTGGCTAGAGGACGCATTCCAGCTAGCTGTTAATGTTAACAGTCCCAGGA
ACAAATCTGAAAATCCAATGGTGCAGCTGTTCTATGGTACTTTCCTGACT
GAAGGGGTTCGTGAAGGAAAACCCTTTTGTAACAATGAGACCTTCGGCCA
GTATCCTCTTCAGGTAAACGGTTATCGCAACTTAGACGAGTGTTTGGAAG
GGGCCATGGTGGAGGGTGATGTTGAGCTTCTTCCCTCCGATCACTCGGTG
AAGTATGGACAAGAGCGTTGGTTTACAAAGCTACCTCCAGTGTTGACCTT
TGAACTCTCAAGATTTGAGTTTAATCAGTCCCTTGGGCAGCCAGAGAAAA
TTCACAATAAGCTGGAATTTCCTCAGATTATTTATATGGACAGGTACATG
TACAGGAGCAAGGAGCTTATTCGAAATAAGAGAGAGTGTATTCGAAAGTT
GAAGGAGGAAATAAAAATTCTGCAGCAAAAATTGGAAAGGTATGTGAAAT
ATGGCTCAGGCCCAGCTCGGTTCCCGCTCCCGGACATGCTGAAATATGTT
ATTGAATTTGCTAGTACAAAACCTGCCTCAGAAAGCTGTCCACCTGAAAG
TGACACACATATGACATTACCACTTTCTTCAGTGCACTGCTCGGTTTCTG
ACCAGACATCCAAGGAAAGTACAAGTACAGAAAGCTCTTCTCAGGATGTT
GAAAGTACCTTTTCTTCTCCTGAAGATTCTTTACCCAAGTCTAAACCACT
GACATCTTCTCGGTCTTCCATGGAAATGCCTTCACAGCCAGCTCCACGAA
CAGTCACAGATGAGGAGATAAATTTTGTTAAGACCTGTCTTCAGAGATGG
AGGAGTGAGATTGAACAAGATATACAAGATTTAAAGACTTGTATTGCAAG
TACTACTCAGACTATTGAACAGATGTACTGCGATCCTCTCCTTCGTCAGG
TAGAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMTAELQQDDAAGAADGHGSSCQMLLNQ
LREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSE
VEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMH
EATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVI
QSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMG
SNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNV
NSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLD
ECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLG
QPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLE
RYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVH
CSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQ
PAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDP
LLRQVE

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12909
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 68685 Daltons