cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pEBG6P-ERK5 T219A Y221F
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK5 T219A Y211F
• Genbank: Q13164
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGC
GGAGCCCCCCGGGCCCGTGAAGGCCGAACCCGCCCACACCGCTGCCTCTG
TAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACC
TTTGACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGC
CTATGGAGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGG
CCATCAAGAAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGG
ACCCTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCAT
CGCCATCAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAAT
CTGTCTACGTGGTCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATC
CACTCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCA
ACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTG
ACCTGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATT
GGTGACTTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCA
GTACTTCATGGCTGAGTTTGTGGCCACGCGCTGGTACCGTGCGCCCGAGC
TCATGCTCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTG
GGCTGCATCTTTGGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAA
AAACTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCAT
CACCAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATC
CAGAGCTTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGG
TGCCGACCGCCAGGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGC
CCAGCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCC
AAGTACCATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTT
TGCCTTTGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTG
TGGCTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAG
ATCCGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCC
AGATGTTGAAATGCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGG
AGTCTCCACCACCAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACC
ATTGATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACC
AAAGAAAGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTG
CCCTGCTCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCC
CTGGAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCG
GGAGCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGG
AGAAACGGCGGCAGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCT
GGGGGCCCCTCCACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGA
CAGAAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAG
CCCTCACCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACC
CCAGTCCAACCTACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGG
CCCGCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTG
CCTGCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCC
ATCCCTGTCCCCGCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGC
TGCCCAGTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAG
GAGTTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGA
GCCCCTCAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCA
GCAGCTATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAG
GCACACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAG
GAATTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGA
TGGCCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGC
TCGAAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAG
ATCCAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGA
CCCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAEPLKEEDGEDG
SAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGN
GAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDN
IIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFL
YQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAE
HQYFMAEFVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFP
GKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVY
PGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPF
DFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPG
CPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPA
PPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQER
QREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSD
NDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQP
TGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSL
LAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLV
TQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGP
QDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDL
QDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12886
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 115752 Daltons