cDNA Clone Ring H2

• Plasmid: pET156P Ring H2
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His Ring H2
• Genbank: AAO72970
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATTCTCTACCAGATGAATTTTTTGTGAGGCATCCTGCTGT
GGAGGATCAGAGGAAGGAAGAAACTGAGAATAAGCTAGAAAAATCATCTG
GTCAACTGAACAAACAGGAAAATGACATACCTACTGATCTTGTCCCTGTT
AACCTACTATTAGAAGTGAAGAAGTTATTAAATGCAATTAATACTCTACC
AAAAGGTGTGGTTCCTCACATTAAGAAGTTCTTACAAGAAGATTTTTCCT
TCCAAACTATGCAGAGAGAAGTTGCAGCTAACAGCCAGAATGGTGAGGAA
ATTGTTCCTGCTTTGACTTTACGTTTCTTGATTACACAGCTAGAAGCAGC
ACTTAGGAACATTCAAGCTGGCAATTATACCGCACACCAGATTAATATTG
GTTATTATTTGACATTACTGTTTTTATATGGAGTAGCACTCACTGAAAGA
GGAAAGAAAGAGGATTATACAGAAGCTGAGAATAAATTTCTGGTGATGAA
GATGATGATCCAAGAAAATGAAATTTGTGAAAACTTTATGTCTTTAGTTT
ATTTTGGACGTGGTTTACTGCGATGTGCTCAAAAGAGATATAATGGAGGA
CTGCTAGAATTTCATAAAAGCTTACAAGAAATTGGAGACAAAAATGACCA
TTGGTTTGACATAGATCCTACAGAAGATGAAGATTTACCTACAACTTTTA
AAGATCTTCTTAATAATTTTATCAAGACAACTGAAAGCAATATAATGAAG
CAGACGATTTGTAGTTACCTAGATTGTGAACGATCTTGTGAAGCTGACAT
TTTGAAGAACACCAGTTATAAGGGATTTTTTCAGTTAATGTGCAGTAAAA
GTTGCTGTGTTTATTTCCATAAAATTTGCTGGAAAAAGTTCAAGAATTTA
AAGTATCCAGAGTTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMDSLPDEFFVRHPAVEDQRKEETENKL
EKSSGQLNKQENDIPTDLVPVNLLLEVKKLLNAINTLPKGVVPHIKKFLQ
EDFSFQTMQREVAANSQNGEEIVPALTLRFLITQLEAALRNIQAGNYTAH
QINIGYYLTLLFLYGVALTERGKKEDYTEAENKFLVMKMMIQENEICENF
MSLVYFGRGLLRCAQKRYNGGLLEFHKSLQEIGDKNDHWFDIDPTEDEDL
PTTFKDLLNNFIKTTESNIMKQTICSYLDCERSCEADILKNTSYKGFFQL
MCSKSCCVYFHKICWKKFKNLKYPEF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12794
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 37684 Daltons