cDNA Clone CBL

• Plasmid: pET156P CBL
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His CBL
• Genbank: P22681
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCGGCAACGTGAAGAAGAGCTCTGGGGCCGGGGGCGGCAG
CGGCTCCGGGGGCTCGGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACG
CCTTCCAGCCGCACCACCACCACCACCACCACCTCAGCCCCCACCCGCCG
GGGACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAA
GGTGGTGCGGTTGTGTCAGAACCCAAAGCTGGCGCTAAAGAATAGCCCAC
CTTATATCTTAGACCTGCTACCAGATACCTACCAGCATCTCCGTACTATC
TTGTCAAGATATGAGGGGAAGATGGAGACACTTGGAGAAAATGAGTATTT
TAGGGTGTTTATGGAGAATTTGATGAAGAAAACTAAGCAAACCATAAGCC
TCTTCAAGGAGGGAAAAGAAAGAATGTATGAGGAGAATTCTCAGCCTAGG
CGAAACCTAACCAAACTGTCCCTCATCTTCAGCCACATGCTGGCAGAACT
AAAAGGAATCTTTCCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTA
CTAAAGCAGATGCTGCGGAATTTTGGAGAAAAGCTTTTGGGGAAAAGACA
ATAGTCCCTTGGAAGAGCTTTCGACAGGCTCTACATGAAGTGCATCCCAT
CAGTTCTGGGCTGGAGGCCATGGCTCTGAAATCCACTATTGATCTGACCT
GCAATGATTATATTTCGGTTTTTGAATTTGACATCTTTACCCGACTCTTT
CAGCCCTGGTCCTCTTTGCTCAGGAATTGGAACAGCCTTGCTGTAACTCA
TCCTGGCTACATGGCTTTTTTGACGTATGACGAAGTGAAAGCTCGGCTCC
AGAAATTCATTCACAAACCTGGCAGTTATATCTTCCGGCTGAGCTGTACT
CGTCTGGGTCAGTGGGCTATTGGGTATGTTACTGCTGATGGGAACATTCT
CCAGACAATCCCTCACAATAAACCTCTCTTCCAAGCACTGATTGATGGCT
TCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAGAATCCTGAT
CTGACTGGCTTATGTGAACCAACTCCCCAAGACCATATCAAAGTGACCCA
GGAACAATATGAATTATACTGTGAGATGGGCTCCACATTCCAACTATGTA
AAATATGTGCTGAAAATGATAAGGATGTAAAGATTGAGCCCTGTGGACAC
CTCATGTGCACATCCTGTCTTACATCCTGGCAGGAATCAGAAGGTCAGGG
CTGTCCTTTCTGCCGATGTGAAATTAAAGGTACTGAACCCATCGTGGTAG
ATCCGTTTGATCCTAGAGGGAGTGGCAGCCTGTTGAGGCAAGGAGCAGAG
GGAGCTCCCTCCCCAAATTATGATGATGATGATGATGAACGAGCTGATGA
TACTCTCTTCATGATGAAGGAATTGGCTGGTGCCAAGGTGGAACGGCCGC
CTTCTCCATTCTCCATGGCCCCACAAGCTTCCCTTCCCCCGGTGCCACCA
CGACTTGACCTTCTGCCGCAGCGAGTATGTGTTCCCTCAAGTGCTTCTGC
TCTTGGAACTGCTTCTAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAAC
CATTGCCAGTACCTCCCACACTTCGAGATCTTCCACCACCACCGCCTCCA
GACCGGCCATATTCTGTTGGAGCAGAATCCCGACCTCAAAGACGCCCCTT
GCCTTGTACACCAGGCGACTGTCCCTCCAGAGACAAACTGCCCCCTGTCC
CCTCTAGCCGCCTTGGAGACTCATGGCTGCCCCGGCCAATCCCCAAAGTA
CCAGTATCTGCCCCAAGTTCCAGTGATCCCTGGACAGGAAGAGAATTAAC
CAACCGGCACTCACTTCCATTTTCATTGCCCTCACAAATGGAGCCCAGAC
CAGATGTGCCTAGGCTCGGAAGCACGTTCAGTCTGGATACCTCCATGAGT
ATGAATAGCAGCCCATTAGTAGGTCCAGAGTGTGACCACCCCAAAATCAA
ACCTTCCTCATCTGCCAATGCCATTTATTCTCTGGCTGCCAGACCTCTTC
CTGTGCCAAAACTGCCACCTGGGGAGCAATGTGAGGGTGAAGAGGACACA
GAGTACATGACTCCCTCTTCCAGGCCTCTACGGCCTTTGGATACATCCCA
GAGTTCACGAGCATGTGATTGCGACCAGCAGATTGATAGCTGTACGTATG
AAGCAATGTATAATATTCAGTCCCAGGCGCCATCTATCACCGAGAGCAGC
ACCTTTGGTGAAGGGAATTTGGCCGCAGCCCATGCCAACACTGGTCCCGA
GGAGTCAGAAAATGAGGATGATGGGTATGATGTCCCAAAGCCACCTGTGC
CGGCCGTGCTGGCCCGCCGAACTCTCTCAGATATCTCTAATGCCAGCTCC
TCCTTTGGCTGGTTGTCTCTGGATGGTGATCCTACAACAAATGTCACTGA
AGGTTCCCAAGTTCCCGAGAGGCCTCCAAAACCATTCCCGCGGAGAATCA
ACTCTGAACGGAAAGCTGGCAGCTGTCAGCAAGGTAGTGGTCCTGCCGCC
TCTGCTGCCACCGCCTCACCTCAGCTCTCCAGTGAGATCGAGAACCTCAT
GAGTCAGGGGTACTCCTACCAGGACATCCAGAAAGCTTTGGTCATTGCCC
AGAACAACATCGAGATGGCCAAAAACATCCTCCGGGAATTTGTTTCCATT
TCTTCTCCTGCCCATGTAGCTACCTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAGNVKKSSGAGGGSGSGGSGSGGLIG
LMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLAL
KNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTK
QTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGD
TFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKST
IDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEV
KARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQA
LIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGST
FQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTE
PIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAK
VERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSL
HKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDK
LPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQ
MEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLA
ARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQID
SCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVP
KPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPF
PRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKA
LVIAQNNIEMAKNILREFVSISSPAHVAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12793
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 102074 Daltons