cDNA Clone UBCH7

• Plasmid: pFBHTb UBCH7
• Parent Plasmid: pFastBac HTb
• Expressed: 6His UBCH7
• Genbank: AAH53368
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCG
CAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATT
TATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAG
GGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACC
ACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAA
AGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCA
ACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCC
CCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGG
ACCGTAAAAAATTCTGTAAGAATGCTGAAGAGTTTACAAAGAAATATGGG
GAAAAGCGACCTGTGGACTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAASRRLMKELEEIRKCGMKNF
RNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKT
KIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLR
ADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12790
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 21232 Daltons