cDNA Clone Ring H2

• Plasmid: pFBHTb Ring H2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His Ring H2
• Genbank: AAO72970
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATTCTCTACCAGATGAATTTTTTGTGAGGCATCCTGCTGT
GGAGGATCAGAGGAAGGAAGAAACTGAGAATAAGCTAGAAAAATCATCTG
GTCAACTGAACAAACAGGAAAATGACATACCTACTGATCTTGTCCCTGTT
AACCTACTATTAGAAGTGAAGAAGTTATTAAATGCAATTAATACTCTACC
AAAAGGTGTGGTTCCTCACATTAAGAAGTTCTTACAAGAAGATTTTTCCT
TCCAAACTATGCAGAGAGAAGTTGCAGCTAACAGCCAGAATGGTGAGGAA
ATTGTTCCTGCTTTGACTTTACGTTTCTTGATTACACAGCTAGAAGCAGC
ACTTAGGAACATTCAAGCTGGCAATTATACCGCACACCAGATTAATATTG
GTTATTATTTGACATTACTGTTTTTATATGGAGTAGCACTCACTGAAAGA
GGAAAGAAAGAGGATTATACAGAAGCTGAGAATAAATTTCTGGTGATGAA
GATGATGATCCAAGAAAATGAAATTTGTGAAAACTTTATGTCTTTAGTTT
ATTTTGGACGTGGTTTACTGCGATGTGCTCAAAAGAGATATAATGGAGGA
CTGCTAGAATTTCATAAAAGCTTACAAGAAATTGGAGACAAAAATGACCA
TTGGTTTGACATAGATCCTACAGAAGATGAAGATTTACCTACAACTTTTA
AAGATCTTCTTAATAATTTTATCAAGACAACTGAAAGCAATATAATGAAG
CAGACGATTTGTAGTTACCTAGATTGTGAACGATCTTGTGAAGCTGACAT
TTTGAAGAACACCAGTTATAAGGGATTTTTTCAGTTAATGTGCAGTAAAA
GTTGCTGTGTTTATTTCCATAAAATTTGCTGGAAAAAGTTCAAGAATTTA
AAGTATCCAGAGTTTTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDSLPDEFFVRHPAVEDQRKEE
TENKLEKSSGQLNKQENDIPTDLVPVNLLLEVKKLLNAINTLPKGVVPHI
KKFLQEDFSFQTMQREVAANSQNGEEIVPALTLRFLITQLEAALRNIQAG
NYTAHQINIGYYLTLLFLYGVALTERGKKEDYTEAENKFLVMKMMIQENE
ICENFMSLVYFGRGLLRCAQKRYNGGLLEFHKSLQEIGDKNDHWFDIDPT
EDEDLPTTFKDLLNNFIKTTESNIMKQTICSYLDCERSCEADILKNTSYK
GFFQLMCSKSCCVYFHKICWKKFKNLKYPEF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 38611 Daltons