cDNA Clone USP28

• Plasmid: pcDNA5-FRT/TO-FLAG USP28 1-582
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: FLAG-USP28
• Genbank: AAH65928.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGACTGCGGAGCTGCAGCAGGACGACGCGGCCGGCGCGGCAGA
CGGCCACGGCTCGAGCTGCCAAATGCTGTTAAATCAACTGAGAGAAATCA
CAGGCATTCAGGACCCTTCCTTTCTCCATGAAGCTCTGAAGGCCAGTAAT
GGTGACATTACTCAGGCAGTCAGCCTTCTCACTGATGAGAGAGTTAAGGA
GCCCAGTCAAGACACTGTTGCTACAGAACCATCTGAAGTAGAGGGGAGTG
CTGCCAACAAGGAAGTATTAGCAAAAGTTATAGACCTTACTCATGATAAC
AAAGATGATCTTCAGGCTGCCATTGCTTTGAGTCTACTGGAGTCTCCCAA
AATTCAAGCTGATGGAAGAGATCTTAACAGGATGCATGAAGCAACCTCTG
CAGAAACTAAACGCTCAAAGAGAAAACGCTGTGAAGTCTGGGGAGAAAAC
CCCAATCCCAATGACTGGAGGAGAGTTGATGGTTGGCCAGTTGGGCTGAA
AAATGTTGGCAATACATGTTGGTTTAGTGCTGTTATTCAGTCTCTCTTTC
AATTGCCTGAATTTCGAAGACTTGTTCTCAGTTATAGTCTGCCACAAAAT
GTACTTGAAAATTGTCGAAGTCATACAGAAAAGAGAAATATCATGTTTAT
GCAAGAGCTTCAGTATTTGTTTGCTCTAATGATGGGATCAAATAGAAAAT
TTGTAGACCCGTCTGCAGCCCTGGATCTATTAAAGGGAGCATTCCGATCA
TCTGAGGAACAGCAGCAAGATGTGAGTGAATTCACACACAAGCTCCTGGA
TTGGCTAGAGGACGCATTCCAGCTAGCTGTTAATGTTAACAGTCCCAGGA
ACAAATCTGAAAATCCAATGGTGCAGCTGTTCTATGGTACTTTCCTGACT
GAAGGGGTTCGTGAAGGAAAACCCTTTTGTAACAATGAGACCTTCGGCCA
GTATCCTCTTCAGGTAAACGGTTATCGCAACTTAGACGAGTGTTTGGAAG
GGGCCATGGTGGAGGGTGATGTTGAGCTTCTTCCCTCCGATCACTCGGTG
AAGTATGGACAAGAGCGTTGGTTTACAAAGCTACCTCCAGTGTTGACCTT
TGAACTCTCAAGATTTGAGTTTAATCAGTCCCTTGGGCAGCCAGAGAAAA
TTCACAATAAGCTGGAATTTCCTCAGATTATTTATATGGACAGGTACATG
TACAGGAGCAAGGAGCTTATTCGAAATAAGAGAGAGTGTATTCGAAAGTT
GAAGGAGGAAATAAAAATTCTGCAGCAAAAATTGGAAAGGTATGTGAAAT
ATGGCTCAGGCCCAGCTCGGTTCCCGCTCCCGGACATGCTGAAATATGTT
ATTGAATTTGCTAGTACAAAACCTGCCTCAGAAAGCTGTCCACCTGAAAG
TGACACACATATGACATTACCACTTTCTTCAGTGCACTGCTCGGTTTCTG
ACCAGACATCCAAGGAAAGTACAAGTACAGAAAGCTCTTCTCAGGATGTT
GAAAGTACCTTTTCTTCTCCTGAAGATTCTTTACCCAAGTCTAAACCACT
GACATCTTCTCGGTCTTCCATGGAAATGCCTTCACAGCCAGCTCCACGAA
CAGTCACAGATGAGGAGATAAATTTTGTTAAGACCTGTCTTCAGAGATGG
AGGAGTGAGATTGAACAAGATATACAAGATTTAAAGACTTGTATTGCAAG
TACTACTCAGACTATTGAACAGATGTACTGCGATCCTCTCCTTCGTCAGG
TAGAATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSF
LHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLA
KVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKR
KRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRL
VLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAAL
DLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMV
QLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDV
ELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFP
QIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARF
PLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKEST
STESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEIN
FVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12777
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 67511 Daltons