cDNA Clone USP28

• Plasmid: pcDNA5-FRT/TO/TAP USP28 1-582
• Parent Plasmid: pCDNA5 FRT/TO TAP
• Expressed: TAP USP28 1-582
• Genbank: AAH65928.1
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCATGACTGCGGAGCTGCAGCAGGACGACGCGGCCGGCGCGGCAGA
CGGCCACGGCTCGAGCTGCCAAATGCTGTTAAATCAACTGAGAGAAATCA
CAGGCATTCAGGACCCTTCCTTTCTCCATGAAGCTCTGAAGGCCAGTAAT
GGTGACATTACTCAGGCAGTCAGCCTTCTCACTGATGAGAGAGTTAAGGA
GCCCAGTCAAGACACTGTTGCTACAGAACCATCTGAAGTAGAGGGGAGTG
CTGCCAACAAGGAAGTATTAGCAAAAGTTATAGACCTTACTCATGATAAC
AAAGATGATCTTCAGGCTGCCATTGCTTTGAGTCTACTGGAGTCTCCCAA
AATTCAAGCTGATGGAAGAGATCTTAACAGGATGCATGAAGCAACCTCTG
CAGAAACTAAACGCTCAAAGAGAAAACGCTGTGAAGTCTGGGGAGAAAAC
CCCAATCCCAATGACTGGAGGAGAGTTGATGGTTGGCCAGTTGGGCTGAA
AAATGTTGGCAATACATGTTGGTTTAGTGCTGTTATTCAGTCTCTCTTTC
AATTGCCTGAATTTCGAAGACTTGTTCTCAGTTATAGTCTGCCACAAAAT
GTACTTGAAAATTGTCGAAGTCATACAGAAAAGAGAAATATCATGTTTAT
GCAAGAGCTTCAGTATTTGTTTGCTCTAATGATGGGATCAAATAGAAAAT
TTGTAGACCCGTCTGCAGCCCTGGATCTATTAAAGGGAGCATTCCGATCA
TCTGAGGAACAGCAGCAAGATGTGAGTGAATTCACACACAAGCTCCTGGA
TTGGCTAGAGGACGCATTCCAGCTAGCTGTTAATGTTAACAGTCCCAGGA
ACAAATCTGAAAATCCAATGGTGCAGCTGTTCTATGGTACTTTCCTGACT
GAAGGGGTTCGTGAAGGAAAACCCTTTTGTAACAATGAGACCTTCGGCCA
GTATCCTCTTCAGGTAAACGGTTATCGCAACTTAGACGAGTGTTTGGAAG
GGGCCATGGTGGAGGGTGATGTTGAGCTTCTTCCCTCCGATCACTCGGTG
AAGTATGGACAAGAGCGTTGGTTTACAAAGCTACCTCCAGTGTTGACCTT
TGAACTCTCAAGATTTGAGTTTAATCAGTCCCTTGGGCAGCCAGAGAAAA
TTCACAATAAGCTGGAATTTCCTCAGATTATTTATATGGACAGGTACATG
TACAGGAGCAAGGAGCTTATTCGAAATAAGAGAGAGTGTATTCGAAAGTT
GAAGGAGGAAATAAAAATTCTGCAGCAAAAATTGGAAAGGTATGTGAAAT
ATGGCTCAGGCCCAGCTCGGTTCCCGCTCCCGGACATGCTGAAATATGTT
ATTGAATTTGCTAGTACAAAACCTGCCTCAGAAAGCTGTCCACCTGAAAG
TGACACACATATGACATTACCACTTTCTTCAGTGCACTGCTCGGTTTCTG
ACCAGACATCCAAGGAAAGTACAAGTACAGAAAGCTCTTCTCAGGATGTT
GAAAGTACCTTTTCTTCTCCTGAAGATTCTTTACCCAAGTCTAAACCACT
GACATCTTCTCGGTCTTCCATGGAAATGCCTTCACAGCCAGCTCCACGAA
CAGTCACAGATGAGGAGATAAATTTTGTTAAGACCTGTCTTCAGAGATGG
AGGAGTGAGATTGAACAAGATATACAAGATTTAAAGACTTGTATTGCAAG
TACTACTCAGACTATTGAACAGATGTACTGCGATCCTCTCCTTCGTCAGG
TAGAATGAGCGGCCGC

Amino Acid Sequence

MDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQS
ANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQS
LKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRR
WKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSMTAELQQDDA
AGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTD
ERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSL
LESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGW
PVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKR
NIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFT
HKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNN
ETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLP
PVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRE
CIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASES
CPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLP
KSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLK
TCIASTTQTIEQMYCDPLLRQVE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12767
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter TAP for mammalian expression.; ; Estimated MW of expressed protein : 87565 Daltons